TY - CHAP
T1 - Old meets new
T2 - Using interspecies interactions to detect secondary metabolite production in actinomycetes
AU - Seyedsayamdost, Mohammad R.
AU - Traxler, Matthew F.
AU - Clardy, Jon
AU - Kolter, Roberto
PY - 2012
Y1 - 2012
N2 - Actinomycetes, a group of filamentous, Gram-positive bacteria, have long been a remarkable source of useful therapeutics. Recent genome sequencing and transcriptomic studies have shown that these bacteria, responsible for half of the clinically used antibiotics, also harbor a large reservoir of gene clusters, which have the potential to produce novel secreted small molecules. Yet, many of these clusters are not expressed under common culture conditions. One reason why these clusters have not been linked to a secreted small molecule lies in the way that actinomycetes have typically been studied: as pure cultures in nutrient-rich media that do not mimic the complex environments in which these bacteria evolved. New methods based on multispecies culture conditions provide an alternative approach to investigating the products of these gene clusters. We have recently implemented binary interspecies interaction assays to mine for new secondary metabolites and to study the underlying biology of interactinomycete interactions. Here, we describe the detailed biological and chemical methods comprising these studies.
AB - Actinomycetes, a group of filamentous, Gram-positive bacteria, have long been a remarkable source of useful therapeutics. Recent genome sequencing and transcriptomic studies have shown that these bacteria, responsible for half of the clinically used antibiotics, also harbor a large reservoir of gene clusters, which have the potential to produce novel secreted small molecules. Yet, many of these clusters are not expressed under common culture conditions. One reason why these clusters have not been linked to a secreted small molecule lies in the way that actinomycetes have typically been studied: as pure cultures in nutrient-rich media that do not mimic the complex environments in which these bacteria evolved. New methods based on multispecies culture conditions provide an alternative approach to investigating the products of these gene clusters. We have recently implemented binary interspecies interaction assays to mine for new secondary metabolites and to study the underlying biology of interactinomycete interactions. Here, we describe the detailed biological and chemical methods comprising these studies.
KW - Amychelin
KW - Amycolatopsis sp. AA4
KW - Interspecies interactions
KW - Secondary metabolites
KW - Streptomyces coelicolor
UR - http://www.scopus.com/inward/record.url?scp=84867632542&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84867632542&partnerID=8YFLogxK
U2 - 10.1016/B978-0-12-404634-4.00005-X
DO - 10.1016/B978-0-12-404634-4.00005-X
M3 - Chapter
C2 - 23084935
AN - SCOPUS:84867632542
SN - 9780124046344
T3 - Methods in Enzymology
SP - 89
EP - 109
BT - Natural Product Biosynthesis by Microorganisms and Plants, Part C
PB - Academic Press Inc.
ER -