TY - JOUR
T1 - Nuclear bodies
T2 - The emerging biophysics of nucleoplasmic phases
AU - Zhu, Lian
AU - Brangwynne, Clifford P.
N1 - Funding Information:
We thank Steph Weber, Shana Elbaum, Nilesh Vaidya, and members of the Brangwynne lab for helpful discussions, and Joseph Gall and Eric Wieschaus for help with the Drosophila experiment shown in Figure 1. This work was supported by the NIH Director's New Innovator Award ( 1DP2GM105437-01 ), the Searle Scholars Program (12-SSP-217) and an NSF CAREER Award ( 1253035 ).
Publisher Copyright:
© 2015.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - The cell nucleus contains a large number of membrane-less bodies that play important roles in the spatiotemporal regulation of gene expression. Recent work suggests that low complexity/disordered protein motifs and repetitive binding domains drive assembly of droplets of nuclear RNA/protein by promoting nucleoplasmic phase separation. Nucleation and maturation of these structures is regulated by, and may in turn affect, factors including post-translational modifications, protein concentration, transcriptional activity, and chromatin state. Here we present a concise review of these exciting recent advances, and discuss current and future challenges in understanding the assembly, regulation, and function of nuclear RNA/protein bodies.
AB - The cell nucleus contains a large number of membrane-less bodies that play important roles in the spatiotemporal regulation of gene expression. Recent work suggests that low complexity/disordered protein motifs and repetitive binding domains drive assembly of droplets of nuclear RNA/protein by promoting nucleoplasmic phase separation. Nucleation and maturation of these structures is regulated by, and may in turn affect, factors including post-translational modifications, protein concentration, transcriptional activity, and chromatin state. Here we present a concise review of these exciting recent advances, and discuss current and future challenges in understanding the assembly, regulation, and function of nuclear RNA/protein bodies.
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U2 - 10.1016/j.ceb.2015.04.003
DO - 10.1016/j.ceb.2015.04.003
M3 - Review article
C2 - 25942753
AN - SCOPUS:84928596005
SN - 0955-0674
VL - 34
SP - 23
EP - 30
JO - Current Opinion in Cell Biology
JF - Current Opinion in Cell Biology
ER -