Novel partitioning of DNA cleavage sites for Drosophila topoisomerase II

Andor Udvardy, Paul Schedl, Miriam Sander, Tao shih Hsieh

Research output: Contribution to journalArticlepeer-review

67 Scopus citations

Abstract

We have examined the long-range distribution of double-stranded DNA cleavage sites for Drosophila melanogaster topoisomerase II. These studies reveal a novel partitioning of preferred topoisomerase II cleavage sites. In the eukaryotic DNAs examined, major cleavage sites were typically found in nontranscribed spacer segments and close to the 5′ and 3′ boundaries of genes. In contrast, there were few if any prominent cleavage sites within genes. In addition, most of the major topoisomerase II cleavage sites closely corresponded to naked DNA hypersensitive sites for the prokaryotic enzyme, micrococcal nuclease.

Original languageEnglish (US)
Pages (from-to)933-941
Number of pages9
JournalCell
Volume40
Issue number4
DOIs
StatePublished - Apr 1985

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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