@article{e3937e43e4464925a64998679e38b71a,
title = "Novel multidomain, multifunctional glycoside hydrolases from highly lignocellulolytic Caldicellulosiruptor species",
abstract = "Biological hydrolysis of microcrystalline cellulose is an uncommon feature in the microbial world, especially among bacteria and archaea growing optimally above 70°C (the so-called extreme thermophiles). In fact, among this group only certain species in the genus Caldicellulosiruptor are capable of rapid and extensive cellulose degradation. Four novel multidomain glycoside hydrolases (GHs) from Caldicellulosiruptor morganii and Caldicellulosiruptor danielii were produced recombinantly in Caldicellulosiruptor bescii and characterized. These GHs are structurally organized with two or three catalytic domains flanking carbohydrate binding modules from Family 3. Collectively, these enzymes represent GH families 5, 9, 10, 12, 44, 48, and 74, and hydrolyze crystalline cellulose, glucan, xylan, and mannan, the primary carbohydrates in plant biomass. Degradation of microcrystalline cellulose by cocktails of GHs from three Caldicellulosiruptor species demonstrated that synergistic interactions enable mixtures of multiple enzymes to outperform single enzymes, suggesting a community mode of action for lignocellulose utilization in thermal environments.",
keywords = "Caldicellulosiruptor, cellulase, glycoside hydrolase, lignocellulose",
author = "Conway, {Jonathan M.} and Crosby, {James R.} and Hren, {Andrew P.} and Southerland, {Robert T.} and Lee, {Laura L.} and Lunin, {Vladimir V.} and Petri Alahuhta and Himmel, {Michael E.} and Bomble, {Yannick J.} and Adams, {Michael W.W.} and Kelly, {Robert M.}",
note = "Funding Information: Funding is provided by the BioEnergy Science Center and the Center for Bioenergy Innovation, from the U.S. Department of Energy (DOE) Bioenergy Research Centers supported by the Office of Biological and Environmental Research in the DOE Office of Science. L. L. Lee acknowledges support from a National Science Foundation Graduate Research Fellowship and a NIH T32 Biotechnology Traineeship (GM008776-11). J. M. Conway and J. R. Crosby acknowledge support from a US DoEd GAANN Fellowship (P200A100004-12 and P200A160061). This work was authored in part by Alliance for Sustainable Energy, LLC, the Manager and Operator of the National Renewable Energy Laboratory for the U.S. Department of Energy under Contract No. DE-AC36-08GO28308. The views expressed in the article do not necessarily represent the views of the DOE or the U.S. Government. The U.S. Government retains, and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work, or allow others to do so, for U.S. Government purposes. Funding Information: Funding is provided by the BioEnergy Science Center and the Center for Bioenergy Innovation, from the U.S. Department of Energy (DOE) Bioenergy Research Centers supported by the Office of Biological and Environmental Research in the DOE Office of Science. L. L. Lee acknowledges support from a National Science Foundation Graduate Research Fellowship and a NIH T32 Biotechnology Traineeship (GM008776-11). J. M. Con-way and J. R. Crosby acknowledge support from a US DoEd GAANN Fellowship (P200A100004-12 and P200A160061). This work was authored in part by Alliance for Sustainable Energy, LLC, the Manager and Operator of the National Renewable Energy Laboratory for the U.S. Department of Energy under Contract No. DE-AC36-08GO28308. The views expressed in the article do not necessarily represent the views of the DOE or the U.S. Government. The U.S. Government retains, and the publisher, by accepting the article for publication, acknowledges that the U.S. Government retains a nonexclusive, paid-up, irrevocable, worldwide license to publish or reproduce the published form of this work, or allow others to do so, for U.S. Government purposes. Publisher Copyright: {\textcopyright} 2018 American Institute of Chemical Engineers",
year = "2018",
month = dec,
doi = "10.1002/aic.16354",
language = "English (US)",
volume = "64",
pages = "4218--4228",
journal = "AICHE Journal",
issn = "0001-1541",
publisher = "American Institute of Chemical Engineers",
number = "12",
}