New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

J. Stader, L. J. Gansheroff, T. J. Silhavy

Research output: Contribution to journalArticle

46 Scopus citations

Abstract

Analysis of more than 100 extragenic suppressors of the lamB14D signal-sequence mutation (changes Val in the hydrophobic core region at position 14 to Asp) has revealed alterations that appear to lie at prlA (secY) and secA (prlD), two loci known to be mutable to suppressor alleles, and a new suppressor termed prlG. One allele of the new suppressor class, prlG1, has been characterized in some detail. This suppressor counteracts, to some degree, the export defect conferred by a variety of signal-sequence mutations in two different genes, lamB and malE. Genetic analysis shows that the dominant suppressor mutations are linked tightly to, and probably allelic with, the gene secE. This result, coupled with data obtained with conditional-lethal alleles of secE, argues strongly that SecE is an important component of the cellular protein export machinery in Escherichia coli.

Original languageEnglish (US)
Pages (from-to)1045-1052
Number of pages8
JournalGenes & development
Volume3
Issue number7
DOIs
StatePublished - Jul 1989

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Fingerprint Dive into the research topics of 'New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.'. Together they form a unique fingerprint.

  • Cite this