TY - JOUR
T1 - Neural-specific elongation of 3′ UTRs during Drosophila development
AU - Hilgers, Valérie
AU - Perry, Michael W.
AU - Hendrix, David
AU - Stark, Alexander
AU - Levine, Michael
AU - Haley, Benjamin
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2011/10/20
Y1 - 2011/10/20
N2 - The 3′ termini of eukaryotic mRNAs influence transcript stability, translation efficiency, and subcellular localization. Here we report that a subset of developmental regulatory genes, enriched in critical RNA-processing factors, exhibits synchronous lengthening of their 3′ UTRs during embryogenesis. The resulting UTRs are up to 20-fold longer than those found on typical Drosophila mRNAs. The large mRNAs emerge shortly after the onset of zygotic transcription, with several of these genes acquiring additional, phased UTR extensions later in embryogenesis. We show that these extended 3′ UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3′ lengthening phenomenon documented in this study. These findings suggest a previously unknown mode of posttranscriptional regulation that may contribute to the complexity of neurogenesis or neural function.
AB - The 3′ termini of eukaryotic mRNAs influence transcript stability, translation efficiency, and subcellular localization. Here we report that a subset of developmental regulatory genes, enriched in critical RNA-processing factors, exhibits synchronous lengthening of their 3′ UTRs during embryogenesis. The resulting UTRs are up to 20-fold longer than those found on typical Drosophila mRNAs. The large mRNAs emerge shortly after the onset of zygotic transcription, with several of these genes acquiring additional, phased UTR extensions later in embryogenesis. We show that these extended 3′ UTR sequences are selectively expressed in neural tissues and contain putative recognition motifs for the translational repressor, Pumilio, which also exhibits the 3′ lengthening phenomenon documented in this study. These findings suggest a previously unknown mode of posttranscriptional regulation that may contribute to the complexity of neurogenesis or neural function.
KW - Alternative polyadenylation
KW - Maternal-to-zygotic transition
KW - Nervous system
KW - Posttranscriptional process
UR - http://www.scopus.com/inward/record.url?scp=80053137344&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053137344&partnerID=8YFLogxK
U2 - 10.1073/pnas.1112672108
DO - 10.1073/pnas.1112672108
M3 - Article
C2 - 21896737
AN - SCOPUS:80053137344
VL - 108
SP - 15864
EP - 15869
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
SN - 0027-8424
IS - 38
ER -