Net nitrogen uptake and DON release in surface waters: Importance of trophic interactions implied from size fractionation experiments

Bettie Ward, D. A. Bronk

Research output: Contribution to journalArticle

42 Scopus citations

Abstract

Factors that influence the release of DON by planktonic assemblages were investigated using size fractionation experiments in the Southern California Bight and in Monterey Bay, California, USA. Incubation experiments, with either 15NH4+ or 15NO3- as a tracer, were used to measure rates of net uptake (incorporation of DIN into particulate nitrogen [PN]) and DON release (production of DON during the incubation, through both active and passive mechanisms). DON release varied greatly among experiments and was higher when 15NO3- was the substrate; it accounted for 3 to nearly 100% of the gross uptake (net uptake plus DON release). Compared with incubations with the <10 μm fraction alone, addition of the <210 μm fraction resulted in distinct patterns of change in net uptake and DON release. Whether the large fraction caused an increase or a decrease in net uptake or DON release rates probably depended on species composition as well as size distribution of trophic groups. DON release rates were positively correlated with NH4+ regeneration rates (p<0.00001) and the magnitude of DON release was about 40 % that of NH4+ regeneration. The results imply that zooplankton and protozoan grazing - on primary producers, small heterotrophic plankton, and possibly bacteria - is a major mechanism of DON release. DON release was observed in every experiment and is clearly an important nitrogen flux in planktonic communities. If grazing is responsible for DON release, then standard incubation experiments probably underestimate the growth of phytoplankton by not accounting for grazing during incubations. The observed rates of DON release imply a rapid turnover of at least a portion of the total DON pool.

Original languageEnglish (US)
Pages (from-to)11-24
Number of pages14
JournalMarine Ecology Progress Series
Volume219
DOIs
StatePublished - Sep 10 2001

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Aquatic Science
  • Ecology

Keywords

  • DON release
  • Grazing
  • Net uptake
  • Size fractionation

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