TY - JOUR
T1 - Nanoparticle targeting of Gram-positive and Gram-negative bacteria for magnetic-based separations of bacterial pathogens
AU - Lu, Hoang D.
AU - Yang, Shirley S.
AU - Wilson, Brian K.
AU - McManus, Simon A.
AU - Chen, Christopher V.H.H.
AU - Prud’homme, Robert K.
N1 - Funding Information:
Acknowledgements We are grateful for support from the Princeton University Center for Health and Wellbeing (HDL), Woodrow Wilson School of Public and International Affairs Program in Science, Technology, and Environmental Policy (HDL). This work was supported by the Princeton University SEAS grant from the Old School Fund (RKP). We thank Prof. Mark Brynildsen for assistance in the bacterial growth experiments. We thank Christina Decoste and John Grady for assistance on flow cytometry experiments.
Funding Information:
We are grateful for support from the Princeton University Center for Health and Wellbeing (HDL), Woodrow Wilson School of Public and International Affairs Program in Science, Technology, and Environmental Policy (HDL). This work was supported by the Princeton University SEAS grant from the Old School Fund (RKP). We thank Prof. Mark Brynildsen for assistance in the bacterial growth experiments. We thank Christina Decoste and John Grady for assistance on flow cytometry experiments.
Publisher Copyright:
© 2017, The Author(s).
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Antimicrobial resistance is a healthcare problem of increasing significance, and there is increasing interest in developing new tools to address bacterial infections. Bacteria-targeting nanoparticles hold promise to improve drug efficacy, compliance, and safety. In addition, nanoparticles can also be used for novel applications, such as bacterial imaging or bioseperations. We here present the use of a scalable block-copolymer-directed self-assembly process, Flash NanoPrecipitation, to form zinc(II)-bis(dipicolylamine) modified nanoparticles that bind to both Gram-positive and Gram-negative bacteria with specificity. Particles have tunable surface ligand densities that change particle avidity and binding efficacy. A variety of materials can be encapsulated into the core of the particles, such as optical dyes or iron oxide colloids, to produce imageable and magnetically active bacterial targeting constructs. As a proof-of-concept, these particles are used to bind and separate bacteria from solution in a magnetic column. Magnetic manipulation and separation would translate to a platform for pathogen identification or removal. These magnetic and targeted nanoparticles enable new methods to address bacterial infections.
AB - Antimicrobial resistance is a healthcare problem of increasing significance, and there is increasing interest in developing new tools to address bacterial infections. Bacteria-targeting nanoparticles hold promise to improve drug efficacy, compliance, and safety. In addition, nanoparticles can also be used for novel applications, such as bacterial imaging or bioseperations. We here present the use of a scalable block-copolymer-directed self-assembly process, Flash NanoPrecipitation, to form zinc(II)-bis(dipicolylamine) modified nanoparticles that bind to both Gram-positive and Gram-negative bacteria with specificity. Particles have tunable surface ligand densities that change particle avidity and binding efficacy. A variety of materials can be encapsulated into the core of the particles, such as optical dyes or iron oxide colloids, to produce imageable and magnetically active bacterial targeting constructs. As a proof-of-concept, these particles are used to bind and separate bacteria from solution in a magnetic column. Magnetic manipulation and separation would translate to a platform for pathogen identification or removal. These magnetic and targeted nanoparticles enable new methods to address bacterial infections.
KW - Antimicrobial resistance
KW - Bacteria
KW - Filtering
KW - Magnetic separations
KW - Nanoparticle
KW - Targeting
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U2 - 10.1007/s13204-017-0548-0
DO - 10.1007/s13204-017-0548-0
M3 - Article
AN - SCOPUS:85020737633
SN - 2190-5509
VL - 7
SP - 83
EP - 93
JO - Applied Nanoscience (Switzerland)
JF - Applied Nanoscience (Switzerland)
IS - 3-4
ER -