Nanoparticle targeting of Gram-positive and Gram-negative bacteria for magnetic-based separations of bacterial pathogens

Hoang D. Lu, Shirley S. Yang, Brian K. Wilson, Simon A. McManus, Christopher V.H.H. Chen, Robert K. Prud’homme

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Antimicrobial resistance is a healthcare problem of increasing significance, and there is increasing interest in developing new tools to address bacterial infections. Bacteria-targeting nanoparticles hold promise to improve drug efficacy, compliance, and safety. In addition, nanoparticles can also be used for novel applications, such as bacterial imaging or bioseperations. We here present the use of a scalable block-copolymer-directed self-assembly process, Flash NanoPrecipitation, to form zinc(II)-bis(dipicolylamine) modified nanoparticles that bind to both Gram-positive and Gram-negative bacteria with specificity. Particles have tunable surface ligand densities that change particle avidity and binding efficacy. A variety of materials can be encapsulated into the core of the particles, such as optical dyes or iron oxide colloids, to produce imageable and magnetically active bacterial targeting constructs. As a proof-of-concept, these particles are used to bind and separate bacteria from solution in a magnetic column. Magnetic manipulation and separation would translate to a platform for pathogen identification or removal. These magnetic and targeted nanoparticles enable new methods to address bacterial infections.

Original languageEnglish (US)
Pages (from-to)83-93
Number of pages11
JournalApplied Nanoscience (Switzerland)
Volume7
Issue number3-4
DOIs
StatePublished - Apr 1 2017

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Atomic and Molecular Physics, and Optics
  • Materials Science (miscellaneous)
  • Physical and Theoretical Chemistry
  • Cell Biology
  • Electrical and Electronic Engineering

Keywords

  • Antimicrobial resistance
  • Bacteria
  • Filtering
  • Magnetic separations
  • Nanoparticle
  • Targeting

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