TY - JOUR
T1 - Mycobactin-mediated iron acquisition within macrophages
AU - Luo, Minkui
AU - Fadeev, Evgeny A.
AU - Groves, John Taylor
N1 - Funding Information:
The authors thank M. Marlow for providing facilities and technical assistance with cell cultures, J. Goodhouse for fluorescent confocal microscopy and F. Morel and B. Ward for iron-labeling experiments. Support of this work by the US National Science Foundation (CHE-0221978) through the Environmental Molecular Science Institute (CEBIC) at Princeton University is gratefully acknowledged.
PY - 2005/8
Y1 - 2005/8
N2 - Restricting the availability of iron is an important strategy for defense against bacterial infection1-3. Mycobacterium tuberculosis survives within the phagosomes of macrophages; consequently, iron acquisition is particularly difficult for M. tuberculosis, because the phagosomal membrane is an additional barrier for its iron access4, 5. Ho wever, little is known about the iron transport and acquisition pathways adapted by this microbe in vivo6. Extracellular iron sources are usually mobilized by hydrophilic siderophores7, 8. Here, we describe direct evidence that mycobactins, the lipophilic siderophores of mycobacteria, efficiently extract intracellular macrophage iron. The metal-free siderophore is diffusely associated with the macrophage membrane, ready for iron chelation. Notably, the mycobactin-metal complex accumulates with high selectivity in macrophage lipid droplets, intracellular domains for lipid storage and sorting9, 10. In our experiments, these mycobactin-targeted lipid droplets were found in direct contact with phagosomes, poised for iron delivery. The existence of this previously undescribed iron acquisition pathway indicates that mycobacteria have taken advantage of endogenous macrophage mechanisms for iron mobilization and lipid sorting for iron acquisition during infection. The pathway could represent a new target for the control of mycobacterial infection.
AB - Restricting the availability of iron is an important strategy for defense against bacterial infection1-3. Mycobacterium tuberculosis survives within the phagosomes of macrophages; consequently, iron acquisition is particularly difficult for M. tuberculosis, because the phagosomal membrane is an additional barrier for its iron access4, 5. Ho wever, little is known about the iron transport and acquisition pathways adapted by this microbe in vivo6. Extracellular iron sources are usually mobilized by hydrophilic siderophores7, 8. Here, we describe direct evidence that mycobactins, the lipophilic siderophores of mycobacteria, efficiently extract intracellular macrophage iron. The metal-free siderophore is diffusely associated with the macrophage membrane, ready for iron chelation. Notably, the mycobactin-metal complex accumulates with high selectivity in macrophage lipid droplets, intracellular domains for lipid storage and sorting9, 10. In our experiments, these mycobactin-targeted lipid droplets were found in direct contact with phagosomes, poised for iron delivery. The existence of this previously undescribed iron acquisition pathway indicates that mycobacteria have taken advantage of endogenous macrophage mechanisms for iron mobilization and lipid sorting for iron acquisition during infection. The pathway could represent a new target for the control of mycobacterial infection.
UR - http://www.scopus.com/inward/record.url?scp=30444432680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=30444432680&partnerID=8YFLogxK
U2 - 10.1038/nchembio717
DO - 10.1038/nchembio717
M3 - Article
C2 - 16408019
AN - SCOPUS:30444432680
SN - 1552-4450
VL - 1
SP - 149
EP - 153
JO - Nature Chemical Biology
JF - Nature Chemical Biology
IS - 3
ER -