Mutations in the exocyst component Sec5 disrupt neuronal membrane traffic, but neurotransmitter release persists

Mala Murthy, Dan Garza, Richard H. Scheller, Thomas L. Schwarz

Research output: Contribution to journalArticlepeer-review

156 Scopus citations

Abstract

The exocyst (Sec6/8) complex is necessary for secretion in yeast and has been postulated to establish polarity by directing vesicle fusion to specific sites along the plasma membrane. The complex may also function in the nervous system, but its precise role is unknown. We have investigated exocyst function in Drosophila with mutations in one member of the complex, sec5. Null alleles die as growth-arrested larvae, whose neuromuscular junctions fail to expand. In culture, neurite outgrowth fails in sec5 mutants once maternal Sec5 is exhausted. Using a trafficking assay, we found impairments in the membrane addition of newly synthesized proteins. In contrast, synaptic vesicle fusion was not impaired. Thus, Sec5 differentiates between two forms of vesicle trafficking: trafficking for cell growth and membrane protein insertion depend on sec5, whereas transmitter secretion does not. In this regard, sec5 differs from the homologs of other yeast exocytosis genes that are required for both neuronal trafficking pathways.

Original languageEnglish (US)
Pages (from-to)433-447
Number of pages15
JournalNeuron
Volume37
Issue number3
DOIs
StatePublished - Feb 6 2003
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)

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