Multiplexed CRISPR-based methods for pathogen nucleic acid detection

Caitlin H. Lamb, Brian Kang, Cameron Myhrvold

Research output: Contribution to journalReview articlepeer-review

2 Scopus citations

Abstract

Bacterial and viral pathogens are devastating to human health and well-being. In many regions, dozens of pathogen species and variants co-circulate. Thus, it is important to detect many different species and variants of pathogens in a given sample through multiplexed detection methods. CRISPR-based nucleic acid detection has shown to be a promising step towards an easy-to-use, sensitive, specific, and high-throughput method to detect nucleic acids from DNA and RNA viruses and bacteria. Here, we review the current state of multiplexed nucleic acid detection methods with a focus on CRISPR-based methods. We also look toward the future of multiplexed point-of-care diagnostics.

Original languageEnglish (US)
Article number100471
JournalCurrent Opinion in Biomedical Engineering
Volume27
DOIs
StatePublished - Sep 2023

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomedical Engineering
  • Medicine (miscellaneous)
  • Biomaterials

Keywords

  • CRISPR
  • Multiplexing
  • Nucleic acid amplification
  • Pathogen detection
  • Point-of-care

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