Multiple sites of alternative splicing of the rat fibronectin gene transcript.

J. E. Schwarzbauer, R. S. Patel, D. Fonda, R. O. Hynes

Research output: Contribution to journalArticle

234 Scopus citations

Abstract

We describe analyses of the structure and expression of the rat fibronectin gene with particular attention to the 40-kb stretch from the center of the gene which encodes 17 type-III repeating units. Each repeat is precisely separated from its neighbors by introns and most are encoded by pairs of exons. Three repeats are encoded precisely by single exons and two of these (EIIIA and EIIIB) are alternatively spliced in a cell type-specific fashion. A third site of alternative splicing (EIIIB) reported here is similar in expression to the previously described EIIIA segment. Both are excluded from mRNA in liver cells and are, therefore, absent from plasma fibronectin. These two alternative splices, plus a third one (V) reported previously, can occur in all possible combinations giving 12 fibronectin mRNAs from a single gene. These splicing variations account for most but not all of the known fibronectin subunit variants. We report investigations designed to detect other regions of alternative splicing. We also show that the pattern of alternative splicing is somewhat altered on oncogenic transformation.

Original languageEnglish (US)
Pages (from-to)2573-2580
Number of pages8
JournalThe EMBO journal
Volume6
Issue number9
DOIs
StatePublished - Sep 1987
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Fingerprint Dive into the research topics of 'Multiple sites of alternative splicing of the rat fibronectin gene transcript.'. Together they form a unique fingerprint.

  • Cite this