@inproceedings{6c0373bea95046d5a0e68bdc67fdb86d,
title = "Multi-wavelength resonance Raman spectroscopy of bacteria to study the effects of growth condition",
abstract = "We will examine the use of multi-wavelength UV resonance-Raman signatures to identify the effects of growth phase on different types of bacteria. Gram positive and gram-negative species, Escherichia coli, Bacillus cereus, Citrobacter koseri and Citrobacter braakii were grown to logarithmic and stationary phases in different culture media. Raman spectra of bacteria were obtained by sequential illumination of samples between 220 and 260 nm; a range which encompasses the resonance frequencies of cellular components. In addition to the information contained in the single spectrum, this two-dimensional signature contains information reflecting variations in resonance cross sections with illumination wavelength. Results of our algorithms in identifying the differences between these germs are discussed. Preliminary results indicate that growth affects the Raman signature, but not to an extent that would negate identification of the species.",
keywords = "Bacteria identification, Multi-wavelength spectroscopy, Resonance Raman spectroscopy, UV",
author = "Nagapratima Kunapareddy and Jacob Grun and Robert Lunsford and David Gillis and Sergei Nikitin and Zheng Wang",
year = "2012",
doi = "10.1117/12.918652",
language = "English (US)",
isbn = "9780819490360",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "SPIE",
booktitle = "Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII",
address = "United States",
note = "Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XIII ; Conference date: 24-04-2012 Through 27-04-2012",
}