TY - JOUR
T1 - MreB polymers and curvature localization are enhanced by RodZ and predict E. coli's cylindrical uniformity
AU - Bratton, Benjamin P.
AU - Shaevitz, Joshua W.
AU - Gitai, Zemer
AU - Morgenstein, Randy M.
N1 - Funding Information:
We thank Thomas Silhavy for the anti-σ70 antibody as well as Nicholas Martin and Robert Scheffler for assistance in Western blotting. We thank Matthias Koch and Elizabeth Ohneck for comments on the manuscript, the members of the Z.G. and J.W.S. laboratories for technical assistance and advice, and Ethan Garner for ongoing discussions on the interactions between MreB and RodZ. This work was supported by National Science Foundation PHY-1734030 (B.P.B., J.W.S.), the Glenn Centers for Aging Research (B.P.B.), National Institutes of Health R01 GM107384 (B.P.B., R.M.M., Z.G.), NIH R21 AI121828 (B.P.B., Z.G., J.W.S.), and NIH F32 GM103290 (R.M.M.). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Publisher Copyright:
© 2018 The Author(s).
PY - 2018/12/1
Y1 - 2018/12/1
N2 - The actin-like protein MreB has been proposed to coordinate the synthesis of the cell wall to determine cell shape in bacteria. MreB is preferentially localized to areas of the cell with specific curved geometries, avoiding the cell poles. It remains unclear whether MreB's curvature preference is regulated by additional factors, and which specific features of MreB promote specific features of rod shape growth. Here, we show that the transmembrane protein RodZ modulates MreB curvature preference and polymer number in E. coli, properties which are regulated independently. An unbiased machine learning analysis shows that MreB polymer number, the total length of MreB polymers, and MreB curvature preference are key correlates of cylindrical uniformity, the variability in radius within a single cell. Changes in the values of these parameters are highly predictive of the resulting changes in cell shape (r 2 = 0.93). Our data thus suggest RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform cylinders.
AB - The actin-like protein MreB has been proposed to coordinate the synthesis of the cell wall to determine cell shape in bacteria. MreB is preferentially localized to areas of the cell with specific curved geometries, avoiding the cell poles. It remains unclear whether MreB's curvature preference is regulated by additional factors, and which specific features of MreB promote specific features of rod shape growth. Here, we show that the transmembrane protein RodZ modulates MreB curvature preference and polymer number in E. coli, properties which are regulated independently. An unbiased machine learning analysis shows that MreB polymer number, the total length of MreB polymers, and MreB curvature preference are key correlates of cylindrical uniformity, the variability in radius within a single cell. Changes in the values of these parameters are highly predictive of the resulting changes in cell shape (r 2 = 0.93). Our data thus suggest RodZ promotes the assembly of geometrically-localized MreB polymers that lead to the growth of uniform cylinders.
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U2 - 10.1038/s41467-018-05186-5
DO - 10.1038/s41467-018-05186-5
M3 - Article
C2 - 30022070
AN - SCOPUS:85050294249
SN - 2041-1723
VL - 9
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 2797
ER -