MreB actin-mediated segregation of a specific region of a bacterial chromosome

Zemer Gitai; Natalie Anne Dye; Ann Reisenauer; Masaaki Wachi; Lucy Shapiro

doi:10.1016/j.cell.2005.01.007

MreB actin-mediated segregation of a specific region of a bacterial chromosome

Zemer Gitai, Natalie Anne Dye, Ann Reisenauer, Masaaki Wachi, Lucy Shapiro

Research output: Contribution to journal › Article › peer-review

323 Scopus citations

Abstract

Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we first demonstrate that MreB is the direct target of the small molecule A22. We then demonstrate that A22 completely blocks the movement of newly replicated loci near the origin of replication but has no qualitative or quantitative effect on the segregation of other loci if added after origin segregation. MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome.

Original language	English (US)
Pages (from-to)	329-341
Number of pages	13
Journal	Cell
Volume	120
Issue number	3
DOIs	https://doi.org/10.1016/j.cell.2005.01.007
State	Published - Feb 11 2005

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

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10.1016/j.cell.2005.01.007

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@article{65df070fc03c473da4fc9792d88de822,

title = "MreB actin-mediated segregation of a specific region of a bacterial chromosome",

abstract = "Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we first demonstrate that MreB is the direct target of the small molecule A22. We then demonstrate that A22 completely blocks the movement of newly replicated loci near the origin of replication but has no qualitative or quantitative effect on the segregation of other loci if added after origin segregation. MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome.",

author = "Zemer Gitai and Dye, {Natalie Anne} and Ann Reisenauer and Masaaki Wachi and Lucy Shapiro",

note = "Funding Information: We would like to thank Tom Wandless (Stanford University) for synthesizing additional A22 compound based on its published structure (Iwai et al., 2002), Patrick McGrath for assistance with the quantitative analysis of locus movement and positioning, and members of the Shapiro and McAdams labs and Coleen Murphy for critical discussions. Z.G. is supported by a Ruth Kirschstein NIH postdoctoral fellowship. This work was supported by NIH grant R01GM51426.",

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doi = "10.1016/j.cell.2005.01.007",

language = "English (US)",

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journal = "Cell",

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T1 - MreB actin-mediated segregation of a specific region of a bacterial chromosome

AU - Gitai, Zemer

AU - Dye, Natalie Anne

AU - Reisenauer, Ann

AU - Wachi, Masaaki

AU - Shapiro, Lucy

N1 - Funding Information: We would like to thank Tom Wandless (Stanford University) for synthesizing additional A22 compound based on its published structure (Iwai et al., 2002), Patrick McGrath for assistance with the quantitative analysis of locus movement and positioning, and members of the Shapiro and McAdams labs and Coleen Murphy for critical discussions. Z.G. is supported by a Ruth Kirschstein NIH postdoctoral fellowship. This work was supported by NIH grant R01GM51426.

PY - 2005/2/11

Y1 - 2005/2/11

N2 - Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we first demonstrate that MreB is the direct target of the small molecule A22. We then demonstrate that A22 completely blocks the movement of newly replicated loci near the origin of replication but has no qualitative or quantitative effect on the segregation of other loci if added after origin segregation. MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome.

AB - Faithful chromosome segregation is an essential component of cell division in all organisms. The eukaryotic mitotic machinery uses the cytoskeleton to move specific chromosomal regions. To investigate the potential role of the actin-like MreB protein in bacterial chromosome segregation, we first demonstrate that MreB is the direct target of the small molecule A22. We then demonstrate that A22 completely blocks the movement of newly replicated loci near the origin of replication but has no qualitative or quantitative effect on the segregation of other loci if added after origin segregation. MreB selectively interacts, directly or indirectly, with origin-proximal regions of the chromosome, arguing that the origin-proximal region segregates via an MreB-dependent mechanism not used by the rest of the chromosome.

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DO - 10.1016/j.cell.2005.01.007

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VL - 120

SP - 329

EP - 341

JO - Cell

JF - Cell

IS - 3

ER -

MreB actin-mediated segregation of a specific region of a bacterial chromosome

Abstract

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