Motor-driven intracellular transport powers bacterial gliding motility

Mingzhai Sun, Morgane Wartel, Eric Cascales, Joshua W. Shaevitz, Tâm Mignot

Research output: Contribution to journalArticle

98 Scopus citations

Abstract

Protein-directed intracellular transport has not been observed in bacteria despite the existence of dynamic protein localization and a complex cytoskeleton. However, protein trafficking has clear potential uses for important cellular processes such as growth, development, chromosome segregation, and motility. Conflicting models have been proposed to explain Myxococcus xanthus motility on solid surfaces, some favoring secretion engines at the rear of cells and others evoking an unknown class of molecular motors distributed along the cell body. Through a combination of fluorescence imaging, force microscopy, and genetic manipulation, we show that membrane-bound cytoplasmic complexes consisting of motor and regulatory proteins are directionally transported down the axis of a cell at constant velocity. This intracellular motion is transmitted to the exterior of the cell and converted to traction forces on the substrate. Thus, this study demonstrates the existence of a conserved class of processive intracellular motors in bacteria and shows how these motors have been adapted to produce cell motility.

Original languageEnglish (US)
Pages (from-to)7559-7564
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume108
Issue number18
DOIs
StatePublished - May 3 2011

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • Murein cluster B
  • Proton motive force

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