TY - JOUR
T1 - Molecular-size determination of xanthan polysaccharide
AU - Prud'homme, Robert K.
AU - Froiman, Gail
AU - Hoagland, David A.
N1 - Funding Information:
We thank those who have invaluably assisted us in this investigation: Dr. Hamish Small of Dow Chemical Company for assistance in h.d.c. techniques, Drs. George Holzwarth and Don Siano of Exxon Central Research Laboratory for assistance on techniques for preparation and characterization of xanthan, Dr. Howard Barth of Hercules Chemical Company for assistance on liquid-chromato,graphy techniques and for providing a summer position for one of us (G-F.). Financial support for this project has been provided by Dow Chemical Company as a fellowship for one of us (D-A-H.), Exxon Central Research Laboratory for capital equipment, and the National Science Foundation (CPESO-03320).
PY - 1982/8/16
Y1 - 1982/8/16
N2 - Hydrodynamic chromatographic separations of xanthan polysaccharide of ultrahigh molecular weight have been obtained by using columns packed with 30-μm, non-porous spheres. From calibration curves of the elution volume versus particle size for spherical, polystyrene latexes, it was found that xanthan is eluted at the same volume as a 0.153-μm diameter sphere. Extremely dilute samples of xanthan (70 p.p.m.) were injected to preclude self-association and aggregate formation. Detection at these low concentrations was accomplished by tagging the xanthan with a fluorescein derivative and using a flow-through fluorometer detector. Flow rates of 1 mL/min yielded run times of ∼7 min. Comparison of the accepted molecular conformation of xanthan-a rigid rod-like molecule-with the apparent molecular volume from the spherical-latex calibration indicates that the xanthan molecules are substantially oriented by the flow field in the chromatography column.
AB - Hydrodynamic chromatographic separations of xanthan polysaccharide of ultrahigh molecular weight have been obtained by using columns packed with 30-μm, non-porous spheres. From calibration curves of the elution volume versus particle size for spherical, polystyrene latexes, it was found that xanthan is eluted at the same volume as a 0.153-μm diameter sphere. Extremely dilute samples of xanthan (70 p.p.m.) were injected to preclude self-association and aggregate formation. Detection at these low concentrations was accomplished by tagging the xanthan with a fluorescein derivative and using a flow-through fluorometer detector. Flow rates of 1 mL/min yielded run times of ∼7 min. Comparison of the accepted molecular conformation of xanthan-a rigid rod-like molecule-with the apparent molecular volume from the spherical-latex calibration indicates that the xanthan molecules are substantially oriented by the flow field in the chromatography column.
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U2 - 10.1016/S0008-6215(00)81076-9
DO - 10.1016/S0008-6215(00)81076-9
M3 - Article
AN - SCOPUS:2542600656
SN - 0008-6215
VL - 106
SP - 225
EP - 233
JO - Carbohydrate Research
JF - Carbohydrate Research
IS - 2
ER -