Molecular imaging of hydrogen peroxide produced for cell signaling

Evan W. Miller; Orapim Tulyanthan; Ehud Y. Isacoff; Christopher J. Chang

doi:10.1038/nchembio871

Molecular imaging of hydrogen peroxide produced for cell signaling

Evan W. Miller, Orapim Tulyanthan, Ehud Y. Isacoff, Christopher J. Chang

Research output: Contribution to journal › Article › peer-review

409 Scopus citations

Abstract

Hydrogen peroxide (H₂O₂) is emerging as a newly recognized messenger in cellular signal transduction. However, a substantial challenge in elucidating its diverse roles in complex biological environments is the lack of methods for probing this reactive oxygen metabolite in living systems with molecular specificity. Here we report the synthesis and application of Peroxy Green 1 (PG1) and Peroxy Crimson 1 (PC1), two new fluorescent probes that show high selectivity for H₂O₂ and are capable of visualizing endogenous H₂O₂ produced in living cells by growth factor stimulation, including the first direct imaging of peroxide produced for brain cell signaling. The combined features of reactive oxygen species selectivity, sensitivity to signaling levels of H₂O ₂, and live-cell compatibility presage many new opportunities for PG1, PC1 and related synthetic reagents for exploring the physiological roles of H₂O₂ in living systems with molecular imaging.

Original language	English (US)
Pages (from-to)	263-267
Number of pages	5
Journal	Nature Chemical Biology
Volume	3
Issue number	5
DOIs	https://doi.org/10.1038/nchembio871
State	Published - May 2007
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology

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abstract = "Hydrogen peroxide (H2O2) is emerging as a newly recognized messenger in cellular signal transduction. However, a substantial challenge in elucidating its diverse roles in complex biological environments is the lack of methods for probing this reactive oxygen metabolite in living systems with molecular specificity. Here we report the synthesis and application of Peroxy Green 1 (PG1) and Peroxy Crimson 1 (PC1), two new fluorescent probes that show high selectivity for H2O2 and are capable of visualizing endogenous H2O2 produced in living cells by growth factor stimulation, including the first direct imaging of peroxide produced for brain cell signaling. The combined features of reactive oxygen species selectivity, sensitivity to signaling levels of H2O 2, and live-cell compatibility presage many new opportunities for PG1, PC1 and related synthetic reagents for exploring the physiological roles of H2O2 in living systems with molecular imaging.",

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Molecular imaging of hydrogen peroxide produced for cell signaling

Abstract

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