Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Arpiar Saunders; Evan Z. Macosko; Alec Wysoker; Melissa Goldman; Fenna M. Krienen; Heather de Rivera; Elizabeth Bien; Matthew Baum; Laura Bortolin; Shuyu Wang; Aleksandrina Goeva; James Nemesh; Nolan Kamitaki; Sara Brumbaugh; David Kulp; Steven A. McCarroll

doi:10.1016/j.cell.2018.07.028

Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Arpiar Saunders, Evan Z. Macosko, Alec Wysoker, Melissa Goldman, Fenna M. Krienen, Heather de Rivera, Elizabeth Bien, Matthew Baum, Laura Bortolin, Shuyu Wang, Aleksandrina Goeva, James Nemesh, Nolan Kamitaki, Sara Brumbaugh, David Kulp, Steven A. McCarroll

Research output: Contribution to journal › Article › peer-review

934 Scopus citations

Abstract

The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.

Original language	English (US)
Pages (from-to)	1015-1030.e16
Journal	Cell
Volume	174
Issue number	4
DOIs	https://doi.org/10.1016/j.cell.2018.07.028
State	Published - Aug 9 2018
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Biochemistry, Genetics and Molecular Biology

Keywords

basal ganglia
brain
single-cell
striatum
transcriptional programs

Access to Document

10.1016/j.cell.2018.07.028

Cite this

Saunders, A., Macosko, E. Z., Wysoker, A., Goldman, M., Krienen, F. M., de Rivera, H., Bien, E., Baum, M., Bortolin, L., Wang, S., Goeva, A., Nemesh, J., Kamitaki, N., Brumbaugh, S., Kulp, D., & McCarroll, S. A. (2018). Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain. Cell, 174(4), 1015-1030.e16. https://doi.org/10.1016/j.cell.2018.07.028

@article{35a1cb454fbc488a98837ce1b9738411,

title = "Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain",

abstract = "The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.",

keywords = "basal ganglia, brain, single-cell, striatum, transcriptional programs",

author = "Arpiar Saunders and Macosko, {Evan Z.} and Alec Wysoker and Melissa Goldman and Krienen, {Fenna M.} and {de Rivera}, Heather and Elizabeth Bien and Matthew Baum and Laura Bortolin and Shuyu Wang and Aleksandrina Goeva and James Nemesh and Nolan Kamitaki and Sara Brumbaugh and David Kulp and McCarroll, {Steven A.}",

note = "Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",

year = "2018",

month = aug,

day = "9",

doi = "10.1016/j.cell.2018.07.028",

language = "English (US)",

volume = "174",

pages = "1015--1030.e16",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "4",

}

TY - JOUR

T1 - Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

AU - Saunders, Arpiar

AU - Macosko, Evan Z.

AU - Wysoker, Alec

AU - Goldman, Melissa

AU - Krienen, Fenna M.

AU - de Rivera, Heather

AU - Bien, Elizabeth

AU - Baum, Matthew

AU - Bortolin, Laura

AU - Wang, Shuyu

AU - Goeva, Aleksandrina

AU - Nemesh, James

AU - Kamitaki, Nolan

AU - Brumbaugh, Sara

AU - Kulp, David

AU - McCarroll, Steven A.

PY - 2018/8/9

Y1 - 2018/8/9

N2 - The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.

AB - The mammalian brain is composed of diverse, specialized cell populations. To systematically ascertain and learn from these cellular specializations, we used Drop-seq to profile RNA expression in 690,000 individual cells sampled from 9 regions of the adult mouse brain. We identified 565 transcriptionally distinct groups of cells using computational approaches developed to distinguish biological from technical signals. Cross-region analysis of these 565 cell populations revealed features of brain organization, including a gene-expression module for synthesizing axonal and presynaptic components, patterns in the co-deployment of voltage-gated ion channels, functional distinctions among the cells of the vasculature and specialization of glutamatergic neurons across cortical regions. Systematic neuronal classifications for two complex basal ganglia nuclei and the striatum revealed a rare population of spiny projection neurons. This adult mouse brain cell atlas, accessible through interactive online software (DropViz), serves as a reference for development, disease, and evolution. Sampling across multiple brain regions identifies hundreds of transcriptionally distinct groups of cells and reveals large-scale features of brain organization and neuronal diversity.

KW - basal ganglia

KW - brain

KW - single-cell

KW - striatum

KW - transcriptional programs

UR - http://www.scopus.com/inward/record.url?scp=85050885385&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85050885385&partnerID=8YFLogxK

U2 - 10.1016/j.cell.2018.07.028

DO - 10.1016/j.cell.2018.07.028

M3 - Article

C2 - 30096299

AN - SCOPUS:85050885385

SN - 0092-8674

VL - 174

SP - 1015-1030.e16

JO - Cell

JF - Cell

IS - 4

ER -

Molecular Diversity and Specializations among the Cells of the Adult Mouse Brain

Abstract

All Science Journal Classification (ASJC) codes

Keywords

Access to Document

Other files and links

Fingerprint

Cite this