Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9

Zhao Yi Wang, Qing Qing Qiu, Wolfgang Seufert, Takahiro Taguchi, Joseph R. Testa, S. A. Whitmore, David F. Callen, Douglas Welsh, Thomas Shenk, Thomas F. Deuel

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Abstract

We report a novel human gone whose product specifically associates with the negative regulatory domain of the Wilms' tumor gene product (WT1) in a yeast two-hybrid screen and with WT in immunoprecipitation and glutathione S- transferase (GST) capture assays. The gone encodes a 17-kDa protein that has 56% amino acid sequence identity with yeast ubiquitin-conjugating enzyme (yUBC) 9, a protein required for cell cycle progression in yeast, and significant identity with other subfamilies of ubiquitin-conjugating enzymes. The human gene fully complements yeast that have a temperature-sensitive yUBC9 gone mutation to fully restore normal growth, indicating that we have cloned a functionally conserved human (h) homolog of yUBC9. Transcripts of hUBC9 of 4.4 kilobases (kb), 2.8 kb, and 1.3 kb were found in all human tissues tested. A single copy of the hUBC9 gene was found and localized to human chromosome 16p13.3. We conclude that hUBC9 retains striking structural and functional conservation with yUBC9 and suggest a possible link of the ubiqaitin/proteosome proteolytic pathway and the WT1 transcriptional repressor system.

Original languageEnglish (US)
Pages (from-to)24811-24816
Number of pages6
JournalJournal of Biological Chemistry
Volume271
Issue number40
DOIs
StatePublished - Oct 15 1996

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Wang, Z. Y., Qiu, Q. Q., Seufert, W., Taguchi, T., Testa, J. R., Whitmore, S. A., Callen, D. F., Welsh, D., Shenk, T., & Deuel, T. F. (1996). Molecular cloning of the cDNA and chromosome localization of the gene for human ubiquitin-conjugating enzyme 9. Journal of Biological Chemistry, 271(40), 24811-24816. https://doi.org/10.1074/jbc.271.40.24811