Molecular characterization of four induced alleles at the Ednrb locus

Myung K. Shin, Liane B. Russell, Shirley M. Tilghman

Research output: Contribution to journalArticle

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Abstract

The piebald locus on mouse chromosome 14 encodes the endothelin-B receptor (EDNRB), a G protein-coupled, seven-transmembrane domain protein, which is required for neural crest-derived melanocyte and enteric neuron development. A spontaneous null allele of Ednrb results in homozygous mice that are predominantly white and die as juveniles from megacolon. To identify the important domains for EDNRB function, four recessive juvenile lethal alleles created by either radiation or chemical mutagens (Ednrb(27Pub), Ednrb(17FrS), Ednrb(1Chlc), and Ednrb(3Chlo)) were examined at the molecular level. Ednrb(27Pub) mice harbor a mutation at a critical proline residue in the fifth transmembrane domain of the EDNRB protein. A gross genomic alteration within the Ednrb gene in Ednrb(3Chlo) results in the production of aberrantly sized transcripts and no authentic Ednrb mRNA. Ednrb(17FrS) mice exhibited a decreased level of Ednrb mRNA, supporting previous observations that the degree of spotting in piebald mice is dependent on the amount of EDNRB expressed. Finally, no molecular defect was detected in Ednrb(1Chlc) mice, which produce normal levels of Ednrb mRNA in adult brain, suggesting that the mutation affects important regulatory elements that mediate the expression of the gene during development.

Original languageEnglish (US)
Pages (from-to)13105-13110
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number24
DOIs
StatePublished - Nov 25 1997

All Science Journal Classification (ASJC) codes

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