Modulations of histone messenger RNA during the early development of Xenopus laevis

J. V. Ruderman; H. R. Woodland; E. A. Sturgess

doi:10.1016/0012-1606(79)90083-6

Modulations of histone messenger RNA during the early development of Xenopus laevis

J. V. Ruderman, H. R. Woodland, E. A. Sturgess

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Abstract

The amount of translatable histone mRNA in Xenopus oocytes, eggs, and embryos was found to be roughly similar, as judged by a cell-free translation assay. This observation provides further evidence that posttranscriptional control mechanisms bring about the 50-fold increase in the rate of histone synthesis during oocyte maturation. About three-quarters of the oocyte histone mRNA was found to be polyadenylated. However, polyadenylated histone mRNA was scarcely detectable in the egg and embryo. Our data are consistent with the speculation presented here that the disappearance of the poly(A)⁺ histone mRNA component at the time of conversion of oocyte to egg (maturation) is due to deadenylation.

Original language	English (US)
Pages (from-to)	71-82
Number of pages	12
Journal	Developmental biology
Volume	71
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(79)90083-6
State	Published - Jul 1979
Externally published	Yes

All Science Journal Classification (ASJC) codes

Molecular Biology
Cell Biology
Developmental Biology

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10.1016/0012-1606(79)90083-6

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title = "Modulations of histone messenger RNA during the early development of Xenopus laevis",

abstract = "The amount of translatable histone mRNA in Xenopus oocytes, eggs, and embryos was found to be roughly similar, as judged by a cell-free translation assay. This observation provides further evidence that posttranscriptional control mechanisms bring about the 50-fold increase in the rate of histone synthesis during oocyte maturation. About three-quarters of the oocyte histone mRNA was found to be polyadenylated. However, polyadenylated histone mRNA was scarcely detectable in the egg and embryo. Our data are consistent with the speculation presented here that the disappearance of the poly(A)+ histone mRNA component at the time of conversion of oocyte to egg (maturation) is due to deadenylation.",

author = "Ruderman, {J. V.} and Woodland, {H. R.} and Sturgess, {E. A.}",

note = "Funding Information: We wish to thank Elizabeth Ballantine and Ruth Ashforth for their help. This work was supported by the Whitaker Health Sciences Fund, the National Institute for Child Health and Human Development (No. HD 10994) (J.V.R.), and the Medical Research Council of Great Britain (H.R.W. and E.A.S.).",

year = "1979",

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AU - Ruderman, J. V.

AU - Woodland, H. R.

AU - Sturgess, E. A.

N1 - Funding Information: We wish to thank Elizabeth Ballantine and Ruth Ashforth for their help. This work was supported by the Whitaker Health Sciences Fund, the National Institute for Child Health and Human Development (No. HD 10994) (J.V.R.), and the Medical Research Council of Great Britain (H.R.W. and E.A.S.).

PY - 1979/7

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N2 - The amount of translatable histone mRNA in Xenopus oocytes, eggs, and embryos was found to be roughly similar, as judged by a cell-free translation assay. This observation provides further evidence that posttranscriptional control mechanisms bring about the 50-fold increase in the rate of histone synthesis during oocyte maturation. About three-quarters of the oocyte histone mRNA was found to be polyadenylated. However, polyadenylated histone mRNA was scarcely detectable in the egg and embryo. Our data are consistent with the speculation presented here that the disappearance of the poly(A)+ histone mRNA component at the time of conversion of oocyte to egg (maturation) is due to deadenylation.

AB - The amount of translatable histone mRNA in Xenopus oocytes, eggs, and embryos was found to be roughly similar, as judged by a cell-free translation assay. This observation provides further evidence that posttranscriptional control mechanisms bring about the 50-fold increase in the rate of histone synthesis during oocyte maturation. About three-quarters of the oocyte histone mRNA was found to be polyadenylated. However, polyadenylated histone mRNA was scarcely detectable in the egg and embryo. Our data are consistent with the speculation presented here that the disappearance of the poly(A)+ histone mRNA component at the time of conversion of oocyte to egg (maturation) is due to deadenylation.

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Modulations of histone messenger RNA during the early development of Xenopus laevis

Abstract

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