Modulating Vibrio cholerae Quorum-Sensing-Controlled Communication Using Autoinducer-Loaded Nanoparticles

Hoang D. Lu, Alina C. Spiegel, Amanda Hurley, Lark J. Perez, Katharina Maisel, Laura M. Ensign, Justin Hanes, Bonnie Lynn Bassler, Martin F. Semmelhack, Robert Krafft Prud'homme

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

The rise of bacterial antibiotic resistance has created a demand for alternatives to traditional antibiotics. Attractive possibilities include pro- and anti-quorum sensing therapies that function by modulating bacterial chemical communication circuits. We report the use of Flash NanoPrecipitation to deliver the Vibrio cholerae quorum-sensing signal CAI-1 ((S)-3-hydroxytridecan-4-one) in a water dispersible form as nanoparticles. The particles activate V. cholerae quorum-sensing responses 5 orders of magnitude higher than does the identically administered free CAI-1 and are diffusive across in vivo delivery barriers such as intestinal mucus. This work highlights the promise of combining quorum-sensing strategies with drug delivery approaches for the development of next-generation medicines.

Original languageEnglish (US)
Pages (from-to)2235-2241
Number of pages7
JournalNano Letters
Volume15
Issue number4
DOIs
StatePublished - Apr 8 2015

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Chemistry(all)
  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering

Keywords

  • CAI-1
  • Nanoparticle
  • Vibrio cholerae
  • autoinducer
  • drug delivery
  • quorum sensing

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