MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells

Kayoko Matsushima, Hajime Isomoto, Naoyuki Yamaguchi, Naoki Inoue, Haruhisa Machida, Toshiyuki Nakayama, Tomayoshi Hayashi, Masaki Kunizaki, Shigekazu Hidaka, Takeshi Nagayasu, Masahiro Nakashima, Kenta Ujifuku, Norisato Mitsutake, Akira Ohtsuru, Shunichi Yamashita, Manav Korpal, Yibin Kang, Philip A. Gregory, Gregory J. Goodall, Shigeru KohnoKazuhiko Nakao

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105 Scopus citations

Abstract

Background: Esophageal squamous cell carcinoma (ESCC) is often diagnosed at later stages until they are incurable. MicroRNA (miR) is a small, non-coding RNA that negatively regulates gene expression mainly via translational repression. Accumulating evidence indicates that deregulation of miR is associated with human malignancies including ESCC. The aim of this study was to identify miR that could be specifically expressed and exert distinct biological actions in ESCC.Methods: Total RNA was extracted from ESCC cell lines, OE21 and TE10, and a non-malignant human esophageal squamous cell line, Het-1A, and subjected to microarray analysis. Expression levels of miR that showed significant differences between the 2 ESCC and Het-1A cells based on the comprehensive analysis were analyzed by the quantitative reverse transcriptase (RT)-PCR method. Then, functional analyses, including cellular proliferation, apoptosis and Matrigel invasion and the wound healing assay, for the specific miR were conducted. Using ESCC tumor samples and paired surrounding non-cancerous tissue obtained endoscopically, the association with histopathological differentiation was examined with quantitative RT-PCR.Results: Based on the miR microarray analysis, there were 14 miRs that showed significant differences (more than 2-fold) in expression between the 2 ESCC cells and non-malignant Het-1A. Among the significantly altered miRs, miR-205 expression levels were exclusively higher in 5 ESCC cell lines examined than any other types of malignant cell lines and Het-1A. Thus, miR-205 could be a specific miR in ESCC. Modulation of miR-205 expression by transfection with its precursor or anti-miR-205 inhibitor did not affect ESCC cell proliferation and apoptosis, but miR-205 was found to be involved in cell invasion and migration. Western blot revealed that knockdown of miR-205 expression in ESCC cells substantially enhanced expression of zinc finger E-box binding homeobox 2, accompanied by reduction of E-cadherin, a regulator of epithelial mesenchymal transition. The miR-205 expression levels were not associated with histological differentiation of human ESCC.Conclusions: These results imply that miR-205 is an ESCC-specific miR that exerts tumor-suppressive activities with EMT inhibition by targeting ZEB2.

Original languageEnglish (US)
Article number30
JournalJournal of Translational Medicine
Volume9
DOIs
StatePublished - Mar 22 2011

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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    Matsushima, K., Isomoto, H., Yamaguchi, N., Inoue, N., Machida, H., Nakayama, T., Hayashi, T., Kunizaki, M., Hidaka, S., Nagayasu, T., Nakashima, M., Ujifuku, K., Mitsutake, N., Ohtsuru, A., Yamashita, S., Korpal, M., Kang, Y., Gregory, P. A., Goodall, G. J., ... Nakao, K. (2011). MiRNA-205 modulates cellular invasion and migration via regulating zinc finger E-box binding homeobox 2 expression in esophageal squamous cell carcinoma cells. Journal of Translational Medicine, 9, [30]. https://doi.org/10.1186/1479-5876-9-30