TY - JOUR
T1 - Microtubule nucleation for spindle assembly
T2 - one molecule at a time
AU - Kraus, Jodi
AU - Alfaro-Aco, Raymundo
AU - Gouveia, Bernardo
AU - Petry, Sabine
N1 - Funding Information:
We would like to thank members of the Petry laboratory, including Michael Rale, Sophie Travis, Venecia Valdez, Bernardo Gouveia, and Aaron Hamlin for their comments and feedback. This work was supported by the Helen Hay Whitney Foundation (for J.K.), the New Innovator Award 1DP2GM123493 and NIGMS grant R01 1R01GM141100-01A1 (to S.P.), as well as those from the Pew Scholars Program in the Biomedical Sciences and the David and Lucile Packard Foundation (both to S.P.).
Publisher Copyright:
© 2023
PY - 2023/9
Y1 - 2023/9
N2 - The cell orchestrates the dance of chromosome segregation with remarkable speed and fidelity. The mitotic spindle is built from scratch after interphase through microtubule (MT) nucleation, which is dependent on the γ-tubulin ring complex (γ-TuRC), the universal MT template. Although several MT nucleation pathways build the spindle framework, the question of when and how γ-TuRC is targeted to these nucleation sites in the spindle and subsequently activated remains an active area of investigation. Recent advances facilitated the discovery of new MT nucleation effectors and their mechanisms of action. In this review, we illuminate each spindle assembly pathway and subsequently consider how the pathways are merged to build a spindle.
AB - The cell orchestrates the dance of chromosome segregation with remarkable speed and fidelity. The mitotic spindle is built from scratch after interphase through microtubule (MT) nucleation, which is dependent on the γ-tubulin ring complex (γ-TuRC), the universal MT template. Although several MT nucleation pathways build the spindle framework, the question of when and how γ-TuRC is targeted to these nucleation sites in the spindle and subsequently activated remains an active area of investigation. Recent advances facilitated the discovery of new MT nucleation effectors and their mechanisms of action. In this review, we illuminate each spindle assembly pathway and subsequently consider how the pathways are merged to build a spindle.
KW - CDK5RAP2
KW - TPX2
KW - XMAP215
KW - augmin
KW - branching MT nucleation
KW - spindle assembly factors (SAFs)
KW - γ-tubulin ring complex (γ-TuRC)
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U2 - 10.1016/j.tibs.2023.06.004
DO - 10.1016/j.tibs.2023.06.004
M3 - Review article
C2 - 37482516
AN - SCOPUS:85165723731
SN - 0968-0004
VL - 48
SP - 761
EP - 775
JO - Trends in Biochemical Sciences
JF - Trends in Biochemical Sciences
IS - 9
ER -