Microfluidic device for label-free measurement of platelet activation

David W. Inglis, Keith J. Morton, John A. Davis, Thomas J. Zieziulewicz, David A. Lawrence, Robert H. Austin, James C. Sturm

Research output: Contribution to journalArticle

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Abstract

In this work we demonstrate a new microfluidic method for the rapid assessment of platelet size and morphology in whole blood. The device continuously fractionates particles according to size by displacing them perpendicularly to the fluid flow direction in a micro-fabricated post array. Whole blood, labeled with the fluorescent, platelet specific, antibody PE-anti-CD41, was run through the device and the positions of fluorescent objects noted as they exited the array. From this, histograms of platelet size were created which show marked increases in size after exposure to thrombin or a temperature of 4°C. We infer that the well known morphological changes that occur during activation are causing the observed increase in size.

Original languageEnglish (US)
Pages (from-to)925-931
Number of pages7
JournalLab on a Chip
Volume8
Issue number6
DOIs
StatePublished - 2008

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biochemistry
  • Chemistry(all)
  • Biomedical Engineering

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  • Cite this

    Inglis, D. W., Morton, K. J., Davis, J. A., Zieziulewicz, T. J., Lawrence, D. A., Austin, R. H., & Sturm, J. C. (2008). Microfluidic device for label-free measurement of platelet activation. Lab on a Chip, 8(6), 925-931. https://doi.org/10.1039/b800721g