Microfabricated arrays of elastomeric posts to study cellular mechanics

Olivia Du Roure, Caroline Dequidt, Alain Richert, Robert H. Austin, Axel Buguin, Philippe Chavrier, Pascal Silberzan, Benoit Ladoux

Research output: Contribution to journalConference articlepeer-review

14 Scopus citations

Abstract

We present an approach to fabricate an array of elastomer posts in order to dynamically measure the traction forces exerted by living cells on a surface with a micrometer lateral resolution. Arrays of closely spaced vertical microposts are made in silicone elastomer (poly(dimethylsiloxane) (PDMS)) by molding a Silicon substrate that has been machined by deep Si etching after standard photolithography. The surface of the micropillars was modified to allow cell culture. Deflections of the calibrated posts were dynamically followed by direct observation with an optical microscope. By using this set-up, we could dynamically draw up a cartography of the local traction forces exerted by the cells.

Original languageEnglish (US)
Pages (from-to)26-34
Number of pages9
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume5345
DOIs
StatePublished - May 10 2004
EventMicrofluidics, BioMEMS, and Medical Microsystems II - San Jose, CA., United States
Duration: Jan 26 2004Jan 27 2004

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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