Abstract
Understanding the metabolic processes associated with aging is key to developing effective management and treatment strategies for age-related diseases. We investigated the metabolic profiles associated with age in a Taiwanese and an American population. 1H NMR spectral profiles were generated for urine specimens collected from the Taiwanese Social Environment and Biomarkers of Aging Study (SEBAS; n = 857; age 54-91 years) and the Mid-Life in the USA study (MIDUS II; n = 1148; age 35-86 years). Multivariate and univariate linear projection methods revealed some common age-related characteristics in urinary metabolite profiles in the American and Taiwanese populations, as well as some distinctive features. In both cases, two metabolites-4-cresyl sulfate (4CS) and phenylacetylglutamine (PAG)-were positively associated with age. In addition, creatine and β-hydroxy-β- methylbutyrate (HMB) were negatively correlated with age in both populations (p < 4 × 10-6). These age-associated gradients in creatine and HMB reflect decreasing muscle mass with age. The systematic increase in PAG and 4CS was confirmed using ultraperformance liquid chromatography-mass spectrometry (UPLC-MS). Both are products of concerted microbial-mammalian host cometabolism and indicate an age-related association with the balance of host-microbiome metabolism.
Original language | English (US) |
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Pages (from-to) | 3166-3180 |
Number of pages | 15 |
Journal | Journal of Proteome Research |
Volume | 12 |
Issue number | 7 |
DOIs | |
State | Published - Jul 5 2013 |
All Science Journal Classification (ASJC) codes
- General Chemistry
- Biochemistry
Keywords
- 4-cresyl sulfate
- NMR spectroscopy
- age
- metabolic profiling
- phenylacetylglutamine
- sex