Methods and Applications of Campenot Trichamber Neuronal Cultures for the Study of Neuroinvasive Viruses

Wesley M. Tierney, Ian A. Vicino, Stella Y. Sun, Wah Chiu, Esteban A. Engel, Matthew P. Taylor, Ian B. Hogue

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

The development of compartmentalized neuron culture systems has been invaluable in the study of neuroinvasive viruses, including the alpha herpesviruses Herpes Simplex Virus 1 (HSV-1) and Pseudorabies Virus (PRV). This chapter provides updated protocols for assembling and culturing rodent embryonic superior cervical ganglion (SCG) and dorsal root ganglion (DRG) neurons in Campenot trichamber cultures. In addition, we provide several illustrative examples of the types of experiments that are enabled by Campenot cultures: (1) Using fluorescence microscopy to investigate axonal outgrowth/extension through the chambers, and alpha herpesvirus infection, intracellular trafficking, and cell-cell spread via axons. (2) Using correlative fluorescence microscopy and cryo electron tomography to investigate the ultrastructure of virus particles trafficking in axons.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages181-206
Number of pages26
DOIs
StatePublished - 2022

Publication series

NameMethods in Molecular Biology
Volume2431
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Alphaherpesvirus
  • Campenot
  • Cryo electron tomography
  • Dorsal root ganglia
  • Fluorescence microscopy
  • Herpes simplex virus
  • Neurons
  • Pseudorabies virus
  • Superior cervical ganglia
  • Virus

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