Metabolomics and Isotope Tracing

Cholsoon Jang, Li Chen, Joshua D. Rabinowitz

Research output: Contribution to journalReview article

110 Scopus citations

Abstract

Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology. A roadmap for measuring metabolite concentrations and using isotope tracers to track metabolic activity.

Original languageEnglish (US)
Pages (from-to)822-837
Number of pages16
JournalCell
Volume173
Issue number4
DOIs
StatePublished - May 3 2018

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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