TY - JOUR
T1 - Metabolomics and Isotope Tracing
AU - Jang, Cholsoon
AU - Chen, Li
AU - Rabinowitz, Joshua D.
N1 - Publisher Copyright:
© 2018 Elsevier Inc.
PY - 2018/5/3
Y1 - 2018/5/3
N2 - Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology. A roadmap for measuring metabolite concentrations and using isotope tracers to track metabolic activity.
AB - Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. Here, we describe the basics of metabolite measurement by MS, including sample preparation, metabolomic analysis, and data interpretation. In addition, drawing on examples of successful experiments, we highlight the ways in which metabolomics and isotope tracing can illuminate biology. A roadmap for measuring metabolite concentrations and using isotope tracers to track metabolic activity.
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U2 - 10.1016/j.cell.2018.03.055
DO - 10.1016/j.cell.2018.03.055
M3 - Review article
C2 - 29727671
AN - SCOPUS:85046167283
SN - 0092-8674
VL - 173
SP - 822
EP - 837
JO - Cell
JF - Cell
IS - 4
ER -