Metabolic promiscuity from the deep subsurface: A story of Survival or Superiority

E. Van Heerden, D. J. Opperman, A. P. Bester, J. Van Marwijk, E. D. Cason, D. Litthauer, L. A. Piater, Tullis C. Onstott

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The Witwatersrand Supergroup is a 2.9-billion-year-old formation of low permeability sandstone and shale with minor volcanic units and conglomerates with an ambient rock temperature of approximately 60°C. Thermus scotoductus SA-01 was isolated from fissure water at a depth of 3.2 kmbls in a South African gold mine and it shows the ability to reduce a variety of heavy metals under anaerobic conditions. It has been postulated that such microorganisms could play an important role in nutrient and metal cycling within the subsurface. Recently, our studies indicate that the cycling of metals could also occur under aerobic conditions and not only by the action of redox active enzymes, but other diverse metabolic proteins as well. In this study the capability of specific proteins to interact with metals is elucidated. Using Thermus SA-01 and its now completed genome sequence, metal reduction is studied through classic proteomic- and genomic methods. Finally we identify thermostable enzymes responsible for the transformation of various metals (Iron, Chrome, Uranium, Gold, etc) and discuss that reduction occurs via the serendipitous action of enzymes with other primary physiological functions, some of which are classical catabolic enzymes and anabolic proteins. This paper discusses the use of a ubiquitous enzyme/protein performing more than one function, possibly detoxifying the environment and using moonlighting as resource to decrease cellular energy requirements rather than elaborate metabolism in the subsurface.

Original languageEnglish (US)
Title of host publicationInstruments, Methods, and Missions for Astrobiology XI
DOIs
StatePublished - Nov 12 2008
EventInstruments, Methods, and Missions for Astrobiology XI - San Diego, CA, United States
Duration: Aug 12 2008Aug 14 2008

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7097
ISSN (Print)0277-786X

Other

OtherInstruments, Methods, and Missions for Astrobiology XI
CountryUnited States
CitySan Diego, CA
Period8/12/088/14/08

All Science Journal Classification (ASJC) codes

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Keywords

  • Chrome
  • Dehydrogenases
  • Gold
  • Iron
  • Metabolic versatility
  • Metal reduction
  • Reductases
  • Thermus scotoductus SA-01
  • Uranium

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  • Cite this

    Van Heerden, E., Opperman, D. J., Bester, A. P., Van Marwijk, J., Cason, E. D., Litthauer, D., Piater, L. A., & Onstott, T. C. (2008). Metabolic promiscuity from the deep subsurface: A story of Survival or Superiority. In Instruments, Methods, and Missions for Astrobiology XI [70970S] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7097). https://doi.org/10.1117/12.801142