Metabolic engineering of Escherichia coli for 1-butanol production

Shota Atsumi; Anthony F. Cann; Michael R. Connor; Claire R. Shen; Kevin M. Smith; Mark P. Brynildsen; Katherine J.Y. Chou; Taizo Hanai; James C. Liao

doi:10.1016/j.ymben.2007.08.003

Metabolic engineering of Escherichia coli for 1-butanol production

Shota Atsumi, Anthony F. Cann, Michael R. Connor, Claire R. Shen, Kevin M. Smith, Mark P. Brynildsen, Katherine J.Y. Chou, Taizo Hanai, James C. Liao

Research output: Contribution to journal › Article › peer-review

702 Scopus citations

Abstract

Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.

Original language	English (US)
Pages (from-to)	305-311
Number of pages	7
Journal	Metabolic Engineering
Volume	10
Issue number	6
DOIs	https://doi.org/10.1016/j.ymben.2007.08.003
State	Published - Nov 2008
Externally published	Yes

All Science Journal Classification (ASJC) codes

Applied Microbiology and Biotechnology
Bioengineering
Biotechnology

Keywords

Biofuel
Butanol
E. coli

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10.1016/j.ymben.2007.08.003

Cite this

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title = "Metabolic engineering of Escherichia coli for 1-butanol production",

abstract = "Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.",

keywords = "Biofuel, Butanol, E. coli",

author = "Shota Atsumi and Cann, {Anthony F.} and Connor, {Michael R.} and Shen, {Claire R.} and Smith, {Kevin M.} and Brynildsen, {Mark P.} and Chou, {Katherine J.Y.} and Taizo Hanai and Liao, {James C.}",

note = "Funding Information: This work was supported by UCLA-DOE Institute for Genomics and Proteomics, and UCLA-NASA CMISE Institute. We are grateful to Hermann Bujard for plasmids. ",

year = "2008",

month = nov,

doi = "10.1016/j.ymben.2007.08.003",

language = "English (US)",

volume = "10",

pages = "305--311",

journal = "Metabolic Engineering",

issn = "1096-7176",

publisher = "Academic Press Inc.",

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TY - JOUR

T1 - Metabolic engineering of Escherichia coli for 1-butanol production

AU - Atsumi, Shota

AU - Cann, Anthony F.

AU - Connor, Michael R.

AU - Shen, Claire R.

AU - Smith, Kevin M.

AU - Brynildsen, Mark P.

AU - Chou, Katherine J.Y.

AU - Hanai, Taizo

AU - Liao, James C.

N1 - Funding Information: This work was supported by UCLA-DOE Institute for Genomics and Proteomics, and UCLA-NASA CMISE Institute. We are grateful to Hermann Bujard for plasmids.

PY - 2008/11

Y1 - 2008/11

N2 - Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.

AB - Compared to ethanol, butanol offers many advantages as a substitute for gasoline because of higher energy content and higher hydrophobicity. Typically, 1-butanol is produced by Clostridium in a mixed-product fermentation. To facilitate strain improvement for specificity and productivity, we engineered a synthetic pathway in Escherichia coli and demonstrated the production of 1-butanol from this non-native user-friendly host. Alternative genes and competing pathway deletions were evaluated for 1-butanol production. Results show promise for using E. coli for 1-butanol production.

KW - Biofuel

KW - Butanol

KW - E. coli

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EP - 311

JO - Metabolic Engineering

JF - Metabolic Engineering

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ER -

Metabolic engineering of Escherichia coli for 1-butanol production

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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