Mechanism of bidirectional thermotaxis in Escherichia coli

Anja Paulick, Vladimir Jakovljevic, Siming Zhang, Michael Erickstad, Alex Groisman, Yigal Meir, William S. Ryu, Ned S. Wingreen, Victor Sourjik

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

In bacteria various tactic responses are mediated by the same cellular pathway, but sensing of physical stimuli remains poorly understood. Here, we combine an in-vivo analysis of the pathway activity with a microfluidic taxis assay and mathematical modeling to investigate the thermotactic response of Escherichia coli. We show that in the absence of chemical attractants E. coli exhibits a steady thermophilic response, the magnitude of which decreases at higher temperatures. Adaptation of wild-type cells to high levels of chemoattractants sensed by only one of the major chemoreceptors leads to inversion of the thermotactic response at intermediate temperatures and bidirectional cell accumulation in a thermal gradient. A mathematical model can explain this behavior based on the saturation-dependent kinetics of adaptive receptor methylation. Lastly, we find that the preferred accumulation temperature corresponds to optimal growth in the presence of the chemoattractant serine, pointing to a physiological relevance of the observed thermotactic behavior.

Original languageEnglish (US)
Article numbere26607
JournaleLife
Volume6
DOIs
StatePublished - Aug 3 2017

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

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