TY - JOUR
T1 - Mechanical stimulation of bioluminescence in the dinoflagellate Gonyaulax polyedra Stein
AU - Anderson, Donald M.
AU - Nosenchuck, Daniel M.
AU - Reynolds, George T.
AU - Walton, Alan J.
N1 - Funding Information:
We thank K. D. Stolzenbach, H.H. Seliger, E. Swift, B. M. Sweeney, P. Botos, Jr. and D. Kulis. The researchw as supported by DOE Contract EY-76-S-02-3120, NSF Grant OCE-8400292, and Ofllce of Naval Research contract N00014-87-K-0007.
PY - 1988/10/27
Y1 - 1988/10/27
N2 - Cultures of the marine dinoflagellate Gonyaulax polyedra Stein were exposed to a variety of flow regimes in small tubes, pressure chambers, and vessels in which objects could be rotated. Bioluminescence was mechanically stimulated by changes in shear, acceleration, and pressure, not by constant values of these parameters. In a biological context, such stimuli would be associated with waves and other surface turbulence, with moving objects such as ships or some large marine organisms, or with close or direct contact as would occur if the dinoflagellate is a prey item. The effects of pressure are complicated by the observation that the luminescence response did not occur in the bulk of the fluid in a pressure chamber, but was confined to the liquid boundaries. The importance of luminescence at surfaces was also seen when objects were rotated in suspensions of G. polyedra; light emissions were restricted to regions with sharp shear gradients. These data were obtained using an image intensifier which made it possible to visualize the spatial pattern of luminescence in the various flow regimes studied. Past results obtained with photo-multipliers are shown to be misleading.
AB - Cultures of the marine dinoflagellate Gonyaulax polyedra Stein were exposed to a variety of flow regimes in small tubes, pressure chambers, and vessels in which objects could be rotated. Bioluminescence was mechanically stimulated by changes in shear, acceleration, and pressure, not by constant values of these parameters. In a biological context, such stimuli would be associated with waves and other surface turbulence, with moving objects such as ships or some large marine organisms, or with close or direct contact as would occur if the dinoflagellate is a prey item. The effects of pressure are complicated by the observation that the luminescence response did not occur in the bulk of the fluid in a pressure chamber, but was confined to the liquid boundaries. The importance of luminescence at surfaces was also seen when objects were rotated in suspensions of G. polyedra; light emissions were restricted to regions with sharp shear gradients. These data were obtained using an image intensifier which made it possible to visualize the spatial pattern of luminescence in the various flow regimes studied. Past results obtained with photo-multipliers are shown to be misleading.
KW - Bioluminescence
KW - Dinoflagellate
KW - Gonyaulax polyedra
KW - Image intensifier
KW - Mechanical stimulation
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U2 - 10.1016/0022-0981(88)90128-1
DO - 10.1016/0022-0981(88)90128-1
M3 - Article
AN - SCOPUS:38249028460
SN - 0022-0981
VL - 122
SP - 277
EP - 288
JO - Journal of Experimental Marine Biology and Ecology
JF - Journal of Experimental Marine Biology and Ecology
IS - 3
ER -