TY - JOUR
T1 - Mcp and Fab-7
T2 - Molecular analysis of putative boundaries of cis-regulatory domains in the bithorax complex of Drosophila melanogaster
AU - Karch, François
AU - Galloni, Mireille
AU - Sipos, Làszlò
AU - Gausz, Janos
AU - Gyurkovics, Henrik
AU - Sched, Paul
N1 - Funding Information:
We would like to thank Nicole Wolff, Jackie Guiard-Maffia, Benjamin Barandun, Clarberta Michelini, Apati Andrasne, Dongo Gyorgyne, Slim Chraiti, and Stella Han for technical assistance. This work was supported in part by the Swiss National Fund (3.193-0.88), the Canton of Geneva, OTKA-920 and NIH. We would especially like to acknowledge and thank the Human Frontier Science Program Organization for a grant which helped make this collaborative research project possible.
PY - 1994/8/11
Y1 - 1994/8/11
N2 - A very large cis-regulatory region of -300 kb is responsible for the complex patterns of expression of the three homeotic genes of the bithorax complex Ubx, abd-A and Abd-B. This region can be subdivided in nine parasegment-specific regulatory subunits. Recent genetic and molecular analysis has revealed the existence of two novel cis-regulatory elements Mcp and Fab-7. Mcp is located between iab-4 and iab-5, the parasegment-specific regulatory subunits which direct Abd-B in parasegments 9 and 10. Similarly, Fab-7 is located between iab-6 and iab-7, the parasegment 11 and 12-specific regulatory units. Mcp and Fab-7 appear to function as domain boundaries that separate adjacent cis-regulatory units. We report the analysis of two new Mcp mutant deletions (Mcp27 and Mcp8116) that allow us to localize sequences essential for boundary function to a - 0 .4 kb DNA segment. These essential sequences closely coincide to a -0.3 kb nuclease hypersensitive region in chromatin. We also show that sequences contributing to the Fab-7 boundary appear to be spread over a larger stretch of DNA, but like Mcp have an unusual chromatin structure.
AB - A very large cis-regulatory region of -300 kb is responsible for the complex patterns of expression of the three homeotic genes of the bithorax complex Ubx, abd-A and Abd-B. This region can be subdivided in nine parasegment-specific regulatory subunits. Recent genetic and molecular analysis has revealed the existence of two novel cis-regulatory elements Mcp and Fab-7. Mcp is located between iab-4 and iab-5, the parasegment-specific regulatory subunits which direct Abd-B in parasegments 9 and 10. Similarly, Fab-7 is located between iab-6 and iab-7, the parasegment 11 and 12-specific regulatory units. Mcp and Fab-7 appear to function as domain boundaries that separate adjacent cis-regulatory units. We report the analysis of two new Mcp mutant deletions (Mcp27 and Mcp8116) that allow us to localize sequences essential for boundary function to a - 0 .4 kb DNA segment. These essential sequences closely coincide to a -0.3 kb nuclease hypersensitive region in chromatin. We also show that sequences contributing to the Fab-7 boundary appear to be spread over a larger stretch of DNA, but like Mcp have an unusual chromatin structure.
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U2 - 10.1093/nar/22.15.3138
DO - 10.1093/nar/22.15.3138
M3 - Article
C2 - 7915032
AN - SCOPUS:0028148117
SN - 0305-1048
VL - 22
SP - 3138
EP - 3146
JO - Nucleic acids research
JF - Nucleic acids research
IS - 15
ER -