Mass spectrometry-based metabolomics of yeast

Christopher A. Crutchfield; Wenyun Lu; Eugene Melamud; Joshua D. Rabinowitz

doi:10.1016/S0076-6879(10)70016-1

Mass spectrometry-based metabolomics of yeast

Christopher A. Crutchfield
, Wenyun Lu
, Eugene Melamud
, Joshua D. Rabinowitz

Research output: Contribution to journal › Article › peer-review

49 Scopus citations

Abstract

Driven by the advent of metabolomics, recent years have seen renewed Interest in the Investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. 2010 Elsevier Inc. All rights reserved.

Original language	English (US)
Pages (from-to)	393-426
Number of pages	34
Journal	Methods in enzymology
Volume	470
Issue number	C
DOIs	https://doi.org/10.1016/S0076-6879(10)70016-1
State	Published - 2010

All Science Journal Classification (ASJC) codes

Biochemistry
Molecular Biology

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10.1016/S0076-6879(10)70016-1

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AU - Lu, Wenyun

AU - Melamud, Eugene

AU - Rabinowitz, Joshua D.

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AB - Driven by the advent of metabolomics, recent years have seen renewed Interest in the Investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. 2010 Elsevier Inc. All rights reserved.

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U2 - 10.1016/S0076-6879(10)70016-1

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SP - 393

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JO - Methods in enzymology

JF - Methods in enzymology

IS - C

ER -

Mass spectrometry-based metabolomics of yeast

Abstract

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