Mass spectrometry-based metabolomics of yeast

Christopher A. Crutchfield, Wenyun Lu, Eugene Melamud, Joshua D. Rabinowitz

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

Driven by the advent of metabolomics, recent years have seen renewed Interest in the Investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. 2010 Elsevier Inc. All rights reserved.

Original languageEnglish (US)
Pages (from-to)393-426
Number of pages34
JournalMethods in enzymology
Volume470
Issue numberC
DOIs
StatePublished - Jan 1 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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