TY - JOUR
T1 - Mass spectrometry-based metabolomics of yeast
AU - Crutchfield, Christopher A.
AU - Lu, Wenyun
AU - Melamud, Eugene
AU - Rabinowitz, Joshua D.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2010
Y1 - 2010
N2 - Driven by the advent of metabolomics, recent years have seen renewed Interest in the Investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. 2010 Elsevier Inc. All rights reserved.
AB - Driven by the advent of metabolomics, recent years have seen renewed Interest in the Investigation of yeast metabolism. Here we provide a practical guide to metabolomic analysis of yeast using liquid chromatography-mass spectrometry (LC-MS). We begin with background on LC-MS and its utility in studying yeast metabolism. We then describe key issues involved at each step of a typical yeast metabolomics experiment: in experimental design, cell culture, metabolite extraction, LC-MS, and data processing and analysis. Throughout, we highlight interdependencies between the steps that are relevant to developing an integrated workflow which effectively leverages LC-MS to reveal yeast biology. 2010 Elsevier Inc. All rights reserved.
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U2 - 10.1016/S0076-6879(10)70016-1
DO - 10.1016/S0076-6879(10)70016-1
M3 - Article
C2 - 20946819
AN - SCOPUS:77956045032
SN - 0076-6879
VL - 470
SP - 393
EP - 426
JO - Methods in enzymology
JF - Methods in enzymology
IS - C
ER -