TY - JOUR
T1 - Mapping social behavior-induced brain activation at cellular resolution in the mouse
AU - Kim, Yongsoo
AU - Venkataraju, Kannan Umadevi
AU - Pradhan, Kith
AU - Mende, Carolin
AU - Taranda, Julian
AU - Turaga, Srinivas C.
AU - Arganda-Carreras, Ignacio
AU - Ng, Lydia
AU - Hawrylycz, Michael J.
AU - Rockland, Kathleen S.
AU - Seung, Hyunjune Sebastian
AU - Osten, Pavel
N1 - Publisher Copyright:
© 2015 The Authors.
PY - 2015/1/13
Y1 - 2015/1/13
N2 - Understanding how brain activation mediates behaviors is a central goal of systems neuroscience. Here, we apply an automated method for mapping brain activation in the mouse in order to probe how sex-specific social behaviors are represented in the male brain. Our method uses the immediate-early-gene c-fos, a marker of neuronal activation, visualized by serial two-photon tomography: the c- fos-GFP+ neurons are computationally detected, their distribution is registered to a reference brain and a brain atlas, and their numbers are analyzed by statistical tests. Our results reveal distinct and shared female and male interaction-evoked patterns of male brain activation representing sex discrimination and social recognition. We also identify brain regions whose degree of activity correlates to specific features of social behaviors and estimate the total numbers and the densities of activated neurons per brain areas. Our study opens the door to automated screening of behavior-evoked brain activation in the mouse.
AB - Understanding how brain activation mediates behaviors is a central goal of systems neuroscience. Here, we apply an automated method for mapping brain activation in the mouse in order to probe how sex-specific social behaviors are represented in the male brain. Our method uses the immediate-early-gene c-fos, a marker of neuronal activation, visualized by serial two-photon tomography: the c- fos-GFP+ neurons are computationally detected, their distribution is registered to a reference brain and a brain atlas, and their numbers are analyzed by statistical tests. Our results reveal distinct and shared female and male interaction-evoked patterns of male brain activation representing sex discrimination and social recognition. We also identify brain regions whose degree of activity correlates to specific features of social behaviors and estimate the total numbers and the densities of activated neurons per brain areas. Our study opens the door to automated screening of behavior-evoked brain activation in the mouse.
UR - http://www.scopus.com/inward/record.url?scp=84920886188&partnerID=8YFLogxK
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U2 - 10.1016/j.celrep.2014.12.014
DO - 10.1016/j.celrep.2014.12.014
M3 - Article
C2 - 25558063
AN - SCOPUS:84920886188
SN - 2211-1247
VL - 10
SP - 292
EP - 305
JO - Cell Reports
JF - Cell Reports
IS - 2
ER -