MAPK Substrate Competition Integrates Patterning Signals in the Drosophila Embryo

Yoosik Kim, Mathieu Coppey, Rona Grossman, Leiore Ajuria, Gerardo Jiménez, Ze'ev Paroush, Stanislav Y. Shvartsman

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

Terminal regions of the Drosophila embryo are patterned by the localized activation of the mitogen-activated protein kinase (MAPK) pathway [1]. This depends on the MAPK-mediated downregulation of Capicua (Cic), a repressor of the terminal gap genes [2, 3]. We establish that downregulation of Cic is antagonized by the anterior patterning morphogen Bicoid (Bcd). We demonstrate that this effect does not depend on transcriptional activity of Bcd and provide evidence suggesting that Bcd, a direct substrate of MAPK, decreases the availability of MAPK for its other substrates, such as Cic. Based on the quantitative analysis of MAPK signaling in multiple mutants, we propose that MAPK substrate competition coordinates the actions of the anterior and terminal patterning systems. In addition, we identify Hunchback as a novel target of MAPK phosphorylation that can account for the previously described genetic interaction between the posterior and terminal systems [4]. Thus, a common enzyme-substrate competition mechanism can integrate the actions of the anterior, posterior, and terminal patterning signals. Substrate competition can be a general signal integration strategy in networks where enzymes, such as MAPK, interact with their multiple regulators and targets [5-10].

Original languageEnglish (US)
Pages (from-to)446-451
Number of pages6
JournalCurrent Biology
Volume20
Issue number5
DOIs
StatePublished - Mar 9 2010

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • SIGNALING

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