TY - JOUR
T1 - Long-term in situ bioelectrochemical monitoring of biohythane process
T2 - Metabolic interactions and microbial evolution
AU - Huang, Sijie
AU - Shen, Mengmeng
AU - Ren, Zhiyong Jason
AU - Wu, Houkai
AU - Yang, Hao
AU - Si, Buchun
AU - Lin, Jianhan
AU - Liu, Zhidan
N1 - Funding Information:
This work was financially supported by the National Natural Science Foundation of China (No. 51861125103 ), the Fundamental Research Funds for the Central Universities, CAU (No. 2020TC160 ) and the Beijing Dairy Industry Innovation Team (No. BAIC06-2021 ).
Publisher Copyright:
© 2021 Elsevier Ltd
PY - 2021/7
Y1 - 2021/7
N2 - Microbial stability and evolution are a critical aspect for biosensors, especially in detecting dynamic and emerging anaerobic biohythane production. In this study, two upflow air–cathode chamber microbial fuel cells (UMFCs) were developed for in situ monitoring of the biohydrogen and biomethane reactors under a COD range of 1000–6000 mg/L and 150–1000 mg/L, respectively. Illumina MiSeq sequencing evidenced the dramatic shift of dominant microbial communities in UMFCs from hydrolytic and acidification bacteria (Clostridiaceae_1, Ruminococcaceae, Peptostreptococcaceae) to acetate-oxidizing bacteria (Synergistaceae, Dysgonomonadaceae, Spirochaetaceae). In addition, exoelectroactive bacteria evaluated from Enterobacteriaceae and Burkholderiaceae to Desulfovibrionaceae and Propionibacteriaceae. Especially, Hydrogenotrophic methanogens (Methanobacteriaceae) were abundant at 93.41% in UMFC (for monitoring hydrogen reactor), which was speculated to be a major metabolic pathway for methane production. Principal component analysis revealed a similarity in microbial structure between UMFCs and methane bioreactors. Microbial network analysis suggested a more stable community structure of UMFCs with 205 days’ operation.
AB - Microbial stability and evolution are a critical aspect for biosensors, especially in detecting dynamic and emerging anaerobic biohythane production. In this study, two upflow air–cathode chamber microbial fuel cells (UMFCs) were developed for in situ monitoring of the biohydrogen and biomethane reactors under a COD range of 1000–6000 mg/L and 150–1000 mg/L, respectively. Illumina MiSeq sequencing evidenced the dramatic shift of dominant microbial communities in UMFCs from hydrolytic and acidification bacteria (Clostridiaceae_1, Ruminococcaceae, Peptostreptococcaceae) to acetate-oxidizing bacteria (Synergistaceae, Dysgonomonadaceae, Spirochaetaceae). In addition, exoelectroactive bacteria evaluated from Enterobacteriaceae and Burkholderiaceae to Desulfovibrionaceae and Propionibacteriaceae. Especially, Hydrogenotrophic methanogens (Methanobacteriaceae) were abundant at 93.41% in UMFC (for monitoring hydrogen reactor), which was speculated to be a major metabolic pathway for methane production. Principal component analysis revealed a similarity in microbial structure between UMFCs and methane bioreactors. Microbial network analysis suggested a more stable community structure of UMFCs with 205 days’ operation.
KW - Anaerobic digestion
KW - Biohythane
KW - Long-term monitoring
KW - Microbial evolution
KW - Microbial fuel cell
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U2 - 10.1016/j.biortech.2021.125119
DO - 10.1016/j.biortech.2021.125119
M3 - Article
C2 - 33848821
AN - SCOPUS:85103941367
SN - 0960-8524
VL - 332
JO - Bioresource Technology
JF - Bioresource Technology
M1 - 125119
ER -