Long-range interactions between transcription factors

Yan Mei Wang; Jonas O. Tegenfeldt; Jim Sturm; Robert H. Austin

doi:10.1088/0957-4484/16/10/003

Long-range interactions between transcription factors

Yan Mei Wang, Jonas O. Tegenfeldt, Jim Sturm, Robert H. Austin

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

We discuss a method for analysing the number of GFP-LacI fusion transcription factors bound to a construct of 256 contiguous LacI binding sites using photon bleaching statics. We show by using a combination of imaging of the construct in nanochannels, photon statistics and addition of IGFP that the binding coefficient of the LacI decreases with increasing occupation of the construct, with a binding coefficient of 10^-6 M when only 15 of the 256 possible sites are occupied. We model this effect by assuming that the GFP-LacI dimer introduces elastic strain into the helix by generalized deformations, and that this strain propagates over distances at least as large as the persistence length.

Original language	English (US)
Pages (from-to)	1993-1999
Number of pages	7
Journal	Nanotechnology
Volume	16
Issue number	10
DOIs	https://doi.org/10.1088/0957-4484/16/10/003
State	Published - Oct 1 2005

All Science Journal Classification (ASJC) codes

General Chemistry
Mechanics of Materials
Mechanical Engineering
Bioengineering
Electrical and Electronic Engineering
General Materials Science

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10.1088/0957-4484/16/10/003

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title = "Long-range interactions between transcription factors",

abstract = "We discuss a method for analysing the number of GFP-LacI fusion transcription factors bound to a construct of 256 contiguous LacI binding sites using photon bleaching statics. We show by using a combination of imaging of the construct in nanochannels, photon statistics and addition of IGFP that the binding coefficient of the LacI decreases with increasing occupation of the construct, with a binding coefficient of 10-6 M when only 15 of the 256 possible sites are occupied. We model this effect by assuming that the GFP-LacI dimer introduces elastic strain into the helix by generalized deformations, and that this strain propagates over distances at least as large as the persistence length.",

author = "Wang, {Yan Mei} and Tegenfeldt, {Jonas O.} and Jim Sturm and Austin, {Robert H.}",

note = "Funding Information: This study was financially supported by National Natural Science Foundation of China (Grant No. 51508228 ), Pearl River S&T Nova Program of Guangzhou, China ( 201710010091 ), China Scholarship Council ( 201706785012 ), Blue Fire Plan ( CXZJHZ201727 ) and the Fundamental Research Funds for the Central Universities, China . ",

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T1 - Long-range interactions between transcription factors

AU - Wang, Yan Mei

AU - Tegenfeldt, Jonas O.

AU - Sturm, Jim

AU - Austin, Robert H.

N1 - Funding Information: This study was financially supported by National Natural Science Foundation of China (Grant No. 51508228 ), Pearl River S&T Nova Program of Guangzhou, China ( 201710010091 ), China Scholarship Council ( 201706785012 ), Blue Fire Plan ( CXZJHZ201727 ) and the Fundamental Research Funds for the Central Universities, China .

PY - 2005/10/1

Y1 - 2005/10/1

N2 - We discuss a method for analysing the number of GFP-LacI fusion transcription factors bound to a construct of 256 contiguous LacI binding sites using photon bleaching statics. We show by using a combination of imaging of the construct in nanochannels, photon statistics and addition of IGFP that the binding coefficient of the LacI decreases with increasing occupation of the construct, with a binding coefficient of 10-6 M when only 15 of the 256 possible sites are occupied. We model this effect by assuming that the GFP-LacI dimer introduces elastic strain into the helix by generalized deformations, and that this strain propagates over distances at least as large as the persistence length.

AB - We discuss a method for analysing the number of GFP-LacI fusion transcription factors bound to a construct of 256 contiguous LacI binding sites using photon bleaching statics. We show by using a combination of imaging of the construct in nanochannels, photon statistics and addition of IGFP that the binding coefficient of the LacI decreases with increasing occupation of the construct, with a binding coefficient of 10-6 M when only 15 of the 256 possible sites are occupied. We model this effect by assuming that the GFP-LacI dimer introduces elastic strain into the helix by generalized deformations, and that this strain propagates over distances at least as large as the persistence length.

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VL - 16

SP - 1993

EP - 1999

JO - Nanotechnology

JF - Nanotechnology

IS - 10

ER -

Long-range interactions between transcription factors

Abstract

All Science Journal Classification (ASJC) codes

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