TY - JOUR
T1 - Localized requirements for windbeutel and pipe reveal a dorsoventral prepattern within the follicular epithelium of the drosophila ovary
AU - Nilson, Laura A.
AU - Schüpbach, Trudi
N1 - Funding Information:
We thank Siegfried Roth for help with initial characterization of dec phenotype in follicle cell clones and for anti-Twi antiserum, Carl Hashimoto for the ndl 111 allele, and Gail Waring for lines containing P[w + , dec + ] transgenes. We also thank Eric Wieschaus, Thomas Vogt, Yashi Ahmed, Jörg Grosshans, and Cheryl VanBuskirk for critical reading of this manuscript. L. A. N. was supported by the Howard Hughes Medical Institute and American Cancer Society postdoctoral fellowship PF-4322. This work was supported by the Howard Hughes Medical Institute and Public Health Service Grant PO1 CA41086 to T. S.
PY - 1998/4/17
Y1 - 1998/4/17
N2 - Establishment of dorsoventral polarity within the Drosophila embryo requires extraembryonic positional information generated during oogenesis. The genes windbeutel, pipe, and nudel are required within the somatic follicle cells of the ovary for production of this spatial cue. Using a novel follicle cell marker system, we have directly evaluated the effect of mutant follicle cell clones on the embryonic dorsoventral pattern. We find no spatially localized requirement for nudel activity. In contrast, windbeutel and pipe are required only within a restricted ventral region of the follicular epithelium. This ventral region can determine lateral embryonic cell fates nonautonomously, indicating that spatial information originating ventrally is subsequently refined, perhaps via diffusion, to yield the gradient of positional information that determines the embryonic dorsoventral pattern.
AB - Establishment of dorsoventral polarity within the Drosophila embryo requires extraembryonic positional information generated during oogenesis. The genes windbeutel, pipe, and nudel are required within the somatic follicle cells of the ovary for production of this spatial cue. Using a novel follicle cell marker system, we have directly evaluated the effect of mutant follicle cell clones on the embryonic dorsoventral pattern. We find no spatially localized requirement for nudel activity. In contrast, windbeutel and pipe are required only within a restricted ventral region of the follicular epithelium. This ventral region can determine lateral embryonic cell fates nonautonomously, indicating that spatial information originating ventrally is subsequently refined, perhaps via diffusion, to yield the gradient of positional information that determines the embryonic dorsoventral pattern.
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U2 - 10.1016/S0092-8674(00)81576-7
DO - 10.1016/S0092-8674(00)81576-7
M3 - Article
C2 - 9568717
AN - SCOPUS:0032540272
SN - 0092-8674
VL - 93
SP - 253
EP - 262
JO - Cell
JF - Cell
IS - 2
ER -