Localized repressors delineate the neurogenic ectoderm in the early Drosophila embryo

Angelike Stathopoulos, Michael Levine

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

The Dorsal gradient produces sequential patterns of gene expression across the dorsoventral axis of early embryos, thereby establishing the presumptive mesoderm, neuroectoderm, and dorsal ectoderm. Spatially localized repressors such as Snail and Vnd exclude the expression of neurogenic genes in the mesoderm and ventral neuroectoderm, respectively. However, no repressors have been identified that establish the dorsal limits of neurogenic gene expression. To investigate this issue, we have conducted an analysis of the ind gene, which is selectively expressed in lateral regions of the presumptive nerve cord. A novel silencer element was identified within the ind enhancer that is essential for eliminating expression in the dorsal ectoderm. Evidence is presented that the associated repressor can function over long distances to silence neighboring enhancers. The ind enhancer also contains a variety of known activator and repressor elements. We propose a model whereby Dorsal and EGF signaling, together with the localized Schnurri repressor, define a broad domain of ind expression throughout the entire presumptive neuroectoderm. The ventral limits of gene expression are defined by the Snail and Vnd repressors, while the dorsal border is established by the newly defined silencer element.

Original languageEnglish (US)
Pages (from-to)482-493
Number of pages12
JournalDevelopmental biology
Volume280
Issue number2
DOIs
StatePublished - Apr 15 2005
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Keywords

  • Dorsal
  • Dorsoventral patterning
  • Drosophila
  • Ind
  • Neurogenic ectoderm
  • Repressor
  • Schnurri
  • Vnd

Fingerprint Dive into the research topics of 'Localized repressors delineate the neurogenic ectoderm in the early Drosophila embryo'. Together they form a unique fingerprint.

  • Cite this