Local chromatin structure at the ribosomal DNA causes replication fork pausing and genome instability in the absence of the S. cerevisiae DNA helicase Rrm3p

Jorge Z. Torres, Jessica B. Bessler, Virginia A. Zakian

Research output: Contribution to journalArticle

55 Scopus citations

Abstract

Lack of the yeast Rrm3p DNA helicase causes replication defects at multiple sites within ribosomal DNA (rDNA), including at the replication fork barrier (RFB). These defects were unaltered in rrm3 sir2 cells. When the RFB binding Foblp was deleted, rrm3-generated defects at the RFB were eliminated, but defects at other rDNA sites were not affected. Thus, specific protein-DNA complexes make replication Rrm3p-dependent. Because rrm3-induced increases in recombination and cell cycle length were only partially suppressed in rrm3 fob1 cells, which still required checkpoint and fork restart activities for viability, non-RFB rrm3-induced defects contribute to rDNA fragility and genome instability.

Original languageEnglish (US)
Pages (from-to)498-503
Number of pages6
JournalGenes and Development
Volume18
Issue number5
DOIs
StatePublished - Mar 1 2004

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Keywords

  • Fob1p
  • Helicase
  • Replication
  • Rrm3p
  • Sir2p
  • rDNA

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