Live imaging of mRNA synthesis in drosophila

Hernan G. Garcia, Thomas Gregor

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

mRNA synthesis is one of the earliest readouts of the activity of a transcribed gene, which is of particular interest in the context of metazoan cell fate specification. These processes are intrinsically dynamic and stochastic, which makes in vivo single-cell measurements inevitable. Here, we present the application of a technology that has been widely used in single celled organisms to measure transcriptional activity in developing embryos of the fruit fly Drosophila melanogaster. The method allows for quantification of instantaneous polymerase occupancy of active gene loci and thereby enables the development and testing of models of gene regulation in development.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages349-357
Number of pages9
DOIs
StatePublished - Jan 1 2018

Publication series

NameMethods in Molecular Biology
Volume1649
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Drosophila
  • Fluorescence
  • Nascent mRNA
  • Quantitative live imaging
  • Transcription activity

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    Garcia, H. G., & Gregor, T. (2018). Live imaging of mRNA synthesis in drosophila. In Methods in Molecular Biology (pp. 349-357). (Methods in Molecular Biology; Vol. 1649). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7213-5_23