TY - JOUR
T1 - Linking diversity and stable isotope fractionation in ammonia-oxidizing bacteria
AU - Casciotti, Karen L.
AU - Sigman, Daniel Mikhail
AU - Ward, Bettie
N1 - Funding Information:
The authors would like to thank Angie Knapp for δ15N analysis of our (NH4)2SO4 reagent, as well as Ruby Ho and Greg Cane for excellent technical assistance. Helpful comments on an earlier version of this manuscript were provided by G. O’Mullan, C. Francis, A. Allen, G. Taroncher-Oldenberg, and L. Chiang. This material is based upon work supported by the National Science Foundation under Grant numbers OCE99-81479 and OCE00-81686 to DMS and OCE96-17690 to BBW, as well as the Center for Environmental Bioinorganic Chemistry (CEBIC), CHE98-10248. KLC was supported by CEBIC and a Harold W. Dodds predoctoral fellowship from Princeton University (2002).
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2003/7
Y1 - 2003/7
N2 - The link between similarity in amino acid sequence,for ammonia monooxygenase (AMO) and isotopic discrimination for ammonia oxidation (εAMO) was investigated in β-subdivision ammonia-oxidizing bacteria. The isotope effects for ammonia oxidation in pure cultures of the nitrifying strains. Nitrosomonas marina, Nitrosomonas C-113a, Nitrosospira tenuis, Nitrosomonas europaea, and Nitrosomonas eutropha ranged from 14.2‰ to 38.2‰. The differences in isotope effects could not be readily explained by differential rates of ammonia oxidation, transport of NH4+, or accumulation of NH2OII or N2O among the strains. The major similarities and differences observed in εAMO are, however, paralleled by similarities and differences in amino acid sequences for the α-subunit of AMO (AmoA). Robust differences in εAMO among nitrifying bacteria may be expected to influence the stable isotopic signatures of nitrous oxide (N2O) produced in various environments.
AB - The link between similarity in amino acid sequence,for ammonia monooxygenase (AMO) and isotopic discrimination for ammonia oxidation (εAMO) was investigated in β-subdivision ammonia-oxidizing bacteria. The isotope effects for ammonia oxidation in pure cultures of the nitrifying strains. Nitrosomonas marina, Nitrosomonas C-113a, Nitrosospira tenuis, Nitrosomonas europaea, and Nitrosomonas eutropha ranged from 14.2‰ to 38.2‰. The differences in isotope effects could not be readily explained by differential rates of ammonia oxidation, transport of NH4+, or accumulation of NH2OII or N2O among the strains. The major similarities and differences observed in εAMO are, however, paralleled by similarities and differences in amino acid sequences for the α-subunit of AMO (AmoA). Robust differences in εAMO among nitrifying bacteria may be expected to influence the stable isotopic signatures of nitrous oxide (N2O) produced in various environments.
KW - Ammonia monooxygenase
KW - Ammonia-oxidizing bacteria
KW - Nitrification
KW - Stable isotope fractionation
UR - http://www.scopus.com/inward/record.url?scp=0141571401&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0141571401&partnerID=8YFLogxK
U2 - 10.1080/01490450303895
DO - 10.1080/01490450303895
M3 - Article
AN - SCOPUS:0141571401
SN - 0149-0451
VL - 20
SP - 335
EP - 353
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 4
ER -