Light-based feedback for controlling intracellular signaling dynamics

Jared E. Toettcher; Delquin Gong; Wendell A. Lim; Orion D. Weiner

doi:10.1038/nmeth.1700

Light-based feedback for controlling intracellular signaling dynamics

Jared E. Toettcher, Delquin Gong, Wendell A. Lim, Orion D. Weiner

Research output: Contribution to journal › Article

160 Scopus citations

Abstract

The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.

Original language	English (US)
Pages (from-to)	837-839
Number of pages	3
Journal	Nature Methods
Volume	8
Issue number	10
DOIs	https://doi.org/10.1038/nmeth.1700
State	Published - Oct 2011
Externally published	Yes

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

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10.1038/nmeth.1700

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Toettcher, J. E., Gong, D., Lim, W. A., & Weiner, O. D. (2011). Light-based feedback for controlling intracellular signaling dynamics. Nature Methods, 8(10), 837-839. https://doi.org/10.1038/nmeth.1700

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