Abstract
The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.
Original language | English (US) |
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Pages (from-to) | 837-839 |
Number of pages | 3 |
Journal | Nature Methods |
Volume | 8 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2011 |
Externally published | Yes |
All Science Journal Classification (ASJC) codes
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology