@article{87061470cb89472795ce79dbfe2d9c65,
title = "Light-based feedback for controlling intracellular signaling dynamics",
abstract = "The ability to apply precise inputs to signaling species in live cells would be transformative for interrogating and understanding complex cell-signaling systems. Here we report an 'optogenetic' method for applying custom signaling inputs using feedback control of a light-gated protein-protein interaction. We applied this strategy to perturb protein localization and phosphoinositide 3-kinase activity, generating time-varying signals and clamping signals to buffer against cell-to-cell variability or changes in pathway activity.",
author = "Toettcher, {Jared E.} and Delquin Gong and Lim, {Wendell A.} and Weiner, {Orion D.}",
note = "Funding Information: We thank all members of the Weiner and Lim laboratories, A. Levskaya, A. Edelstein and N. Stuurman for helpful comments and discussions, and A. Loewer and H. El-Samad for critically reading this manuscript. TagBFP construct was a gift from members of the Vale lab (University of California San Francisco), pHR lentiviral vectors were provided by J.R. James (University of California San Francisco), and the iSH construct was a gift from members of the Meyer lab (Stanford University). This work was supported by a Cancer Research Institute Postdoctoral Fellowship to J.E.T., an American Cancer Society postdoctoral fellowship to D.G., the Howard Hughes Medical Institute and US National Institutes of Health grants GM55040, GM62583, EY016546 and P50GM081879 to W.A.L., and a National Institutes of Health grant GM084040 and a Searle Scholars Fellowship to O.D.W.",
year = "2011",
month = oct,
doi = "10.1038/nmeth.1700",
language = "English (US)",
volume = "8",
pages = "837--839",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "10",
}