Ligand dynamics in the photodissociation of carboxyhemoglobin by subpicosecond transient infrared spectroscopy

L. Rothberg, T. M. Jedju, R. H. Austin

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Time-resolved infrared spectroscopy with 0.5-ps resolution is used to track the evolution of the CO stretching vibration after visible photoexcitation of carboxyhemoglobin in water at room temperature. Polarization measurements determine that the iron-complexed CO is oriented nearly perpendicular to the porphyrin plane. The dissociation appears to proceed via a metastable excited state with 2 +/- 1 ps lifetime. The dissociated CO binds weakly in the heme pocket for at least 500 ps. This state correlates with the internally bound state observed by Frauenfelder et al. at low temperatures in myoglobin.

Original languageEnglish (US)
Pages (from-to)369-373
Number of pages5
JournalBiophysical Journal
Volume57
Issue number2
DOIs
StatePublished - 1990

All Science Journal Classification (ASJC) codes

  • Biophysics

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