Ligand-Binding Basics: Evaluating Intermolecular Affinity, Specificity, Stoichiometry, and Cooperativity

A concise and accessible textbook covering ligand-binding theory in chemistry, biology, and drug development. In Ligand-binding Basics: Evaluating Intermolecular Affinity, Specificity, Stoichiometry, and Cooperativity, accomplished chemist Professor Jannette Carey introduces ligand binding in a thorough and practical way for those new to the topic, as well as anyone seeking a connection between theory and experiment. Using a minimum of mathematical formalism, this book offers analytical rigor while remaining accessible to non-specialist practitioners. It provides readers with the skills they need to analyze their own binding data or published results, helping them develop an intuitive grasp of ligand-binding phenomena integrated with structural and thermodynamic understanding. Topics covered include: Application of the principles of equilibrium, mass action, and mass balance to derive the basic equations that describe all binding processes Recommended approaches for plotting and graphical analysis of binding data Strategies for designing, analyzing, interpreting, and troubleshooting experiments from the perspective of ligand-binding theory. Review of selected examples that illustrate integration of structural and thermodynamic analysis. Perfect for students and educators in chemistry, biochemistry, molecular biology, and pharmaceutical science, Ligand-binding Basics will also appeal to practitioners who aim to study ligand binding in any molecular system.