TY - JOUR
T1 - LC-MS data processing with MAVEN
T2 - A metabolomic analysis and visualization engine
AU - Clasquin, Michelle F.
AU - Melamud, Eugene
AU - Rabinowitz, Joshua D.
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/3
Y1 - 2012/3
N2 - MAVEN is an open-source software program for interactive processing of LC-MS-based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high-resolution full-scan mass spectrometry data. It automatically detects and reports peak intensities for isotope-labeled metabolites. Menu-driven, click-based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step-by-step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope-labeling patterns, and export of tables of metabolite-specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC-MS data into a form ready for biological analysis.
AB - MAVEN is an open-source software program for interactive processing of LC-MS-based metabolomics data. MAVEN enables rapid and reliable metabolite quantitation from multiple reaction monitoring data or high-resolution full-scan mass spectrometry data. It automatically detects and reports peak intensities for isotope-labeled metabolites. Menu-driven, click-based navigation allows visualization of raw and analyzed data. Here we provide a User Guide for MAVEN. Step-by-step instructions are provided for data import, peak alignment across samples, identification of metabolites that differ strongly between biological conditions, quantitation and visualization of isotope-labeling patterns, and export of tables of metabolite-specific peak intensities. Together, these instructions describe a workflow that allows efficient processing of raw LC-MS data into a form ready for biological analysis.
KW - Kinetic flux profiling
KW - Liquid chromatography-mass spectrometry
KW - Metabolic flux analysis
KW - Metabolomics
KW - Pathway visualization and mapping
KW - Stable isotope labeling
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U2 - 10.1002/0471250953.bi1411s37
DO - 10.1002/0471250953.bi1411s37
M3 - Article
C2 - 22389014
AN - SCOPUS:84860009126
SN - 1934-3396
JO - Current Protocols in Bioinformatics
JF - Current Protocols in Bioinformatics
IS - SUPPL.37
M1 - 14.11
ER -